Abstract
Zebrafish is convenient for transient genetic manipulations such as morpholino-mediated gene knockdown, DNA/mRNA-based genetic overexpression. These genetic approaches do not involve the challenge of making transgenic animals, and their effect can last up to a week. This protocol describes the detailed steps of injecting to zebrafish embryos.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol was modified from the original protocol created and developed by V. Schneider in the Michael Granato lab, University of Pennsylvania, Philadelphia, USA, and supported by NIH grant R01HD037975.
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