Abstract
The sedimentation rate of a protein in a linear sucrose gradient can be used to determine its S value when compared to proteins of known S values which are run in a parallel gradient. This can be used to estimate an approximate molecular weight of the protein and test the potential interaction of two proteins if they peak in the same gradient range.
Keywords: Sucrose gradient, Molecular weight of the protein, S value
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was developed in the laboratory of Dr. Guowei Fang (Department of Biology, Stanford University, Stanford, CA, USA). This work was supported by a Burroughs-Wellcome Career Award in Biomedical Research (G.F.) and by grants from National Institutes of Health (GM062852 to G.F.).
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.
Yes, You are right:10% sucrose solutions: 10 g sucrose in 100 ml ddH2O40% sucrose solutions: 40 g sucrose in 100 ml ddH2OThanks.