Abstract
This protocol explains how to extract DNA from a single zebrafish embryo. It does not require the use of expensive kits.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was developed in the Michael Granato Lab at University of Pennsylvania, Philadelphia, USA, and this work was supported by NIH grant R01HD037975.
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Hi,This happened with one of my primer pair too but worked by adjusting the annealing temperature. It might be due to high salts.Good luck,Ajay
Thank you :) I will try to adjust annealing temp.