Abstract
In situ hybridization is routinely used to examine the gene expression level and location of embryos. This protocol is modified from the Thisse protocol and is a detailed description of the in situ hybridization procedures in zebrafish embryos.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was modified from Reference 1, and developed in the Michael Granato Lab at University of Pennsylvania, Philadelphia, USA. This work was supported by NIH grant R01HD037975.
References
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