Abstract
Growth of cells in the subcutaneous space of immunocompromised mice is a common method for assaying tumorigenic potential in vivo. This technique is also used to assess the effects of therapeutic interventions on cancer cell lines.
Materials and Reagents
Equipment
Procedure
Recipes
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Matrigel has been shown to significantly enhance the tumorigenicity of a wide range of cancer cell lines in vivo.https://link.springer.com/protocol/10.1385/1-59259-406-9:287if you don't want to use matrigel, you should use large number of cells for increased tumorigenicity of cancer cells.
Thank you so much for your response.
You should be consistent between, but I expect the experiment will work. It should also be acknowledged that tumor growth may depend on serum and medium.
In which way may the tumor growth depend on the serum? Will not it be drain out if it is in small volum(100ul)? Thx
The fluid will be absorbed. However, it may support the cells initially. My only point is that you haven't strictly tested the cells' ability to grow tumors independent of additional supplements.
ok. Thank you very much for your advice and quick reply.