Abstract
This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from Reference 1. Funding from the NIH supported this work.
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.
Since this is a well developed and relatively common protocol, there is no reference for this protocol. But if you would like explore more literature to help your experiment, you could check the manuals of related products from commercial vendor, such as Qiagen, Pierce, GE etc, which I often found helpful. Here are some examples: https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314787424814/litdoc28962284AA_20110831144559.pdf http://www.piercenet.com/instructions/2160671.pdf Hope this helps.