Abstract
The EmulsiFlex-C3 homogenizer is powered by an electric motor. The pump does not require a compressor for it to run. This equipment can be used to disrupt cells at a large scale. The EmulsiFlex-C3 has a fixed flow-through capacity of 3 L/h. It has the ability to process samples as small as 10 ml. The homogenizing pressure is adjustable between 500 and 30,000 psi. In this protocol, we describe the use of the Avestin Emulsiflex C3 Homogenizer to disrupt S. pombe and S. cerevisiae cells.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol has been modified and adapted in the Espenshade Lab, Johns Hopkins School of Medicine. Funding to support different projects that have used this protocol has come from NIH – National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases, the Pancreatic Cancer Action Network, and the American Heart Association.
References
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Hello Mathew,It shouldn't make a difference with compressed air or nitrogen. However, consult with the manufacturer first.Zongtian