实验原理：根据叶绿素提取液对可见光谱的吸收，利用分光光度计在特定波长下测定叶绿素提取物的吸光值，然后根据公式计算出样品中叶绿素的含量。实验目的：可以检测水稻叶片中叶绿素a、叶绿素b及总叶绿素含量。
关键词: 叶绿素a, 叶绿素b, 总叶绿素含量
材料与试剂
仪器设备
实验步骤
结果与分析
根据Arnon法 (1949) 的公式加以修正计算叶绿素含量：叶绿素a = (12.72A663-2.59A645) × v/w × 1,000叶绿素b = (22.88A645-4.67A663) × v/w × 1,000叶绿素总含量 = (20.29A645+8.05A663) × v/w × 1,000
注意事项
溶液配方
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