实验原理:样品在Buffer A作用下细胞破裂,释放胞浆蛋白及其他蛋白,离心后所得沉淀中包含细胞核,再经高盐Buffer抽提可得到核蛋白。 实验目的:抽提核蛋白可用于PAGE电泳,Western blot,免疫共沉淀等下游实验,可排除胞质蛋白及其他蛋白的影响。
关键词: 核蛋白抽提, 高盐buffer, 低盐buffer, 透析
材料与试剂
注:所有试剂尽量用sigma进口试剂。
仪器设备
实验步骤
注意事项
溶液配方
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