实验原理:CTAB (hexadecyltrimethylammonium bromide,十六烷基三甲基溴化铵),是一种阳离子去污剂,可溶解细胞膜,并与核酸形成复合物。该复合物在高盐溶液中 (>0.7 mol/L NaCl) 可溶,并可稳定存在。通过有机溶剂抽提,去除蛋白、多糖、酚类等杂质后加入乙醇沉淀即可使核酸分离出来。 实验目的:快速提取植物基因组DNA,可用于PCR检测,对转基因植株进行初步分子鉴定。
关键词: CTAB, DNA小样抽提, PCR
材料与试剂
仪器设备
实验步骤
注意事项
溶液配方
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