材料与试剂

1. 100 mm Cell Culture Dish with Clear Lid, Sterile (NEST, catalog number: 704001)
2. CellCarrier-96 Black, Optically Clear Bottom, Tissue Culture Treated (PerkinElmer, catalog number: 6005550)
3. Poly-D-Lysine (PDL) (Beyotime, catalog number: C0312)
4. Hoechst 33342 (Beyotime, catalog number: C1022, stored at -80 °C)
5. DMEM medium (Hyclone, catalog number: SH30243.01, stored at 4 °C)
6. Fetal Bovine Serum (Biological Industries, catalog number: 04-001-1ACS, stored at -80 °C)
7. Penicillin-streptomycin (ThermoFisher, catalog number: 15140148, stored at 4 °C)
8. Decitabine (Selleck, catalog number: S1200, aliquoted and stored at -80 °C)
9. DMSO (MP Biomedicals, catalog number: 196055)
10. 1x DPBS (ThermoFisher, catalog number: 14190144)
11. Trypsin-Versene mixture (Lonza, catalog number: 17-161E, stored at 4 °C)
12. Trypan Blue 0.04% solution (Gibco, catalog number: 15250061)
13. DMEM培养基 (见溶液配方)
14. PDL溶液 (见溶液配方)
15. Hoechst 33342工作溶液 (见溶液配方)
    

仪器设备

1. 二氧化碳细胞培养箱 (Memmert, CO2 incubator ICO50)
2. 无菌超净工作台 (ESCO, model: AC2-4S1)
3. 水平转子低速离心机 (ThermoFisher, model: Labofuge 400)
4. 八连排移液枪 (ThermoFisher, model: 14-386-352N))
5. Bright Line Hemocytometer (Weber Scientific, model: 3048-11)
6. Opera Phenix High-Content Screening System (PerkinElmer, model: HH14000000)
   

实验步骤