Detection of Flavonoids in Different Tissues from Different Population of Citrus Fruits by LC-MS

材料与试剂

1. 色谱柱 (shim-packVP-ODS C18 (pore size 5.0 μm, length 2 x 150 mm))
   
2. 内标 (Lidocaine)
   
3. 类黄酮标准品 (apigenin 6-C-glucoside, apigenin 7-O-glucoside, luteolin 7-O-glucoside等)
   
4. 乙酸
   
5. 甲醇 (色谱纯)
   
6. 乙腈 (色谱纯)
   
7. 超纯水
   

仪器设备

1. Milli-Q s ULTRA purification system (Millipore, Milford, MA, USA)
   
2. HPLC-ESI-QqTOF-MS/MS (用于定性分析，靶向MS²模式)
   
3. LC-ESI-Q TRAP-MS/MS (用于定量分析，MRM模式)

实验步骤