Immunology

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Self-Paced Five-Choice Serial Reaction Time-Task for Mouse Behavioral Testing

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    Sample Preparation and Integrative Data Analysis of a Droplet-based Single-Cell ATAC-sequencing Using Murine Thymic Epithelial Cells
    Authors:  Tatsuya Ishikawa, Hiroto Ishii, Takahisa Miyao, Kenta Horie, Maki Miyauchi, Nobuko Akiyama and Taishin Akiyama, date: 01/05/2023, view: 467, Q&A: 0

    Accessible chromatin regions modulate gene expression by acting as cis-regulatory elements. Understanding the epigenetic landscape by mapping accessible regions of DNA is therefore imperative to decipher mechanisms of gene regulation under

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    Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
    Authors:  Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd, date: 09/05/2020, view: 4985, Q&A: 0
    Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate ...
    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 7717, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    Detection of Intracellular Reduced (Catalytically Active) SHP-1 and Analyses of Catalytically Inactive SHP-1 after Oxidation by Pervanadate or H2O2
    Authors:  Seeyoung Choi and Paul E. Love, date: 01/05/2018, view: 5737, Q&A: 0
    Oxidative inactivation of cysteine-dependent Protein Tyrosine Phosphatases (PTPs) by cellular reactive oxygen species (ROS) plays a critical role in regulating signal transduction in multiple cell types. The phosphatase activity of most PTPs depends ...
    Measurement of Dipeptidylpeptidase Activity in vitro and in vivo
    Authors:  Rosa Barreira da Silva, Molly A. Ingersoll and Matthew L. Albert, date: 03/20/2017, view: 7881, Q&A: 0
    Dipeptidylpeptidases (DPPs) are serine proteases, which cleave small proteins and peptides possessing a proline or an alanine in the second position of their N-terminus. Among the members of this family, dipeptidylpeptidase 4 (DPP4) is ...
    In vitro Treatment of Mouse and Human Cells with Endogenous Ligands for Activation of the Aryl Hydrocarbon Receptor
    Authors:  Taisho Yamada and Akinori Takaoka, date: 01/05/2017, view: 7641, Q&A: 0
    Activation of the aryl hydrocarbon receptor (AHR) by endogenous ligands has been implicated in a variety of physiological processes such as cell cycle regulation, cell differentiation and immune responses. It is reported that tryptophan metabolites, ...
    Determination of H2O2 Generation by pHPA Assay
    Authors:  Jennifer L. Larson-Casey and A. Brent Carter, date: 11/20/2016, view: 8214, Q&A: 0
    The production of reactive oxygen species, including H2O2, is a process that can be used in signaling, cell death, or immune response. To quantify oxidative stress in cells, a fluorescence technique has been modified from a ...
    Reconstruction of the Mouse Inflammasome System in HEK293T Cells
    Authors:  Hexin Shi, Anne Murray and Bruce Beutler, date: 11/05/2016, view: 10456, Q&A: 0
    The NLRP3 (NLR family, Pyrin domain containing 3) inflammasome is a multiprotein complex comprised of NLRP3, pro-caspase-1, the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), and the protein kinase NIMA related ...
    Quantification of Tumor Material Uptake
    Authors:  Richard N. Hanna and Catherine C. Hedrick, date: 10/20/2016, view: 8140, Q&A: 0
    Extracellular tumor material including exosomes, microvesicles and apoptotic tumor debris may help cancers invade new organs. Enhancing the removal of extracellular tumor material by immune cells represents a novel immunotherapy approach for ...
    In vivo Imaging of Tumor and Immune Cell Interactions in the Lung
    Authors:  Richard N. Hanna, Grzegorz Chodaczek and Catherine C. Hedrick, date: 10/20/2016, view: 9015, Q&A: 0
    Immunotherapy has demonstrated great therapeutic potential by activating the immune system to fight cancer. However, little is known about the specific dynamics of interactions that occur between tumor and immune cells. In this protocol we describe ...



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