Measuring Intracellular H2O2 in Intact Human Cells Using the Genetically Encoded Fluorescent Sensor HyPer7
    Authors:  Lianne J. H. C. Jacobs, Michaela N. Hoehne and Jan Riemer, date: 10/20/2022, view: 746, Q&A: 0

    Depending on its local concentration, hydrogen peroxide (H2O2) can serve as a cellular signaling molecule but can also cause damage to biomolecules. The levels of H2O2 are influenced by the activity of its generator sites, local antioxidative

    H2O2 Release Assay
    Authors:  Santosh Yadav, Shruthi Sanjitha Sampath, Brian J. Deskin and Victor J. Thannickal, date: 06/05/2022, view: 1826, Q&A: 0

    Reactive oxygen species are ubiquitous in nature, and function as signalling molecules in biological systems; they may also contribute to oxidative stress in several pathobiological disease states. In this report, we describe a simple, reliable,

    Amplex Red Assay for Measuring Hydrogen Peroxide Production from Caenorhabditis elegans
    Authors:  Ozgur Karakuzu, Melissa R. Cruz, Yi Liu and Danielle A. Garsin, date: 11/05/2019, view: 9412, Q&A: 0
    Reagents such as Amplex® Red have been developed for detecting hydrogen peroxide (H2O2) and are used to measure the release of H2O2 from biological samples such as mammalian leukocytes ...
    Quantifying MAMP-induced Production of Reactive Oxygen Species in Sorghum and Maize
    Plants have the ability to recognize microbe-associated molecular patterns (MAMPs) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, a ...
    Hypochlorous Acid Staining with R19-S in the Drosophila Intestine upon Ingestion of Opportunistic Bacteria
    Authors:  Salma Hachfi, Olivia Benguettat and Armel Gallet, date: 05/20/2019, view: 4949, Q&A: 0
    The intestine is endowed with an innate immune system that is required to fight any exogenous bacteria that are swallowed along with the food. The first line of defense that is mounted by the gut epithelium is the release of immune Reactive Oxygen ...
    Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2’,7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA)
    Authors:  Rajneesh, Jainendra Pathak, Ananya Chatterjee, Shailendra P. Singh and Rajeshwar P. Sinha, date: 09/05/2017, view: 20844, Q&A: 0
    Reactive oxygen species (ROS) are cell signaling molecules synthesized inside the cells as a response to routine metabolic processes. In stress conditions such as ultraviolet radiation (UVR), ROS concentration increases several folds in the cells ...
    Measuring Oxidative Stress in Caenorhabditis elegans: Paraquat and Juglone Sensitivity Assays
    Authors:  Megan M. Senchuk, Dylan J. Dues and Jeremy M. Van Raamsdonk, date: 01/05/2017, view: 12599, Q&A: 0
    Oxidative stress has been proposed to be one of the main causes of aging and has been implicated in the pathogenesis of many diseases. Sensitivity to oxidative stress can be measured by quantifying survival following exposure to a reactive oxygen ...
    Detection of Reactive Oxygen Species in Oryza sativa L. (Rice)
    Authors:  Navdeep Kaur, Isha Sharma, Kamal Kirat and Pratap Kumar Pati, date: 12/20/2016, view: 19126, Q&A: 2
    Superoxide ions (O2-) and hydrogen peroxide (H2O2) are the reactive oxygen species (ROS) that play a significant role in regulation of many plant processes. The level of O2- ions is determined ...
    Hydrogen Peroxide Measurement in Arabidopsis Root Tissue Using Amplex Red
    Authors:  Tzvetina Brumbarova, Cham Thi Tuyet Le and Petra Bauer, date: 11/05/2016, view: 14137, Q&A: 0
    This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. When root tissue is disrupted and ...
    In vitro Real-time Measurement of the Intra-bacterial Redox Potential
    Authors:  Joris van der Heijden and B. Brett Finlay, date: 09/05/2015, view: 8473, Q&A: 0
    All bacteria that live in oxygenated environments have to deal with oxidative stress caused by some form of exogenous or endogenous reactive oxygen species (ROS) (Imlay, 2013). Large quantities of ROS damage DNA, lipids and proteins which can ...

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