Immunology

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Self-Paced Five-Choice Serial Reaction Time-Task for Mouse Behavioral Testing

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    An Optimized Tat/Rev Induced Limiting Dilution Assay for the Characterization of HIV-1 Latent Reservoirs
    Authors:  Swarnima Mishra, Yuvrajsinh Gohil, Kavita Mehta, Anish D'silva, Afzal Amanullah, Deepak Selvam, Neelam Pargain, Narendra Nala, G. N. Sanjeeva and Udaykumar Ranga, date: 04/20/2022, view: 1277, Q&A: 0

    The administration of antiretroviral therapy (ART) leads to a rapid reduction in plasma viral load in HIV-1 seropositive subjects. However, when ART is suspended, the virus rebounds due to the presence of a latent viral reservoir. Several techniques

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    Suppression of Human Dendritic Cells by Regulatory T Cells
    Authors:  Qing Huang, Avery J. Lam, Dominic A. Boardman, Nicholas A. J. Dawson and Megan K. Levings, date: 11/05/2021, view: 2686, Q&A: 0

    Regulatory T cells (Tregs) suppress immune responses via a variety of mechanisms and can be used as a cellular therapy to induce tolerance. The function of Tregs is commonly assessed in vitro using assays that measure suppression of effector T cell

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    In vitro Differentiation of Thymic Treg Cell Progenitors to Mature Thymic Treg Cells
    Authors:  David L. Owen and Michael A. Farrar, date: 08/20/2019, view: 3519, Q&A: 0
    Thymic Treg cell differentiation occurs via a two-step process. Step one generates Treg cell progenitors (TregP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated ...
    Ultrasound Guided Intra-thymic Injection to Track Recent Thymic Emigrants and Investigate T Cell Development
    Authors:  Haiguang Wang, David L. Owen, Lily J. Qian, Laura B. Chopp, Michael A. Farrar and Kristin A. Hogquist, date: 12/05/2018, view: 4157, Q&A: 0
    To track recent thymic emigrants (RTEs) or study T cell development in the thymus, intra-thymic injection of a cellular tag or precursor cells for various T cell lineages is often desired. However, the traditional surgical approach to expose the ...
    Generation of Busulfan Chimeric Mice for the Analysis of T Cell Population Dynamics
    Authors:  Thea Hogan, Andrew Yates and Benedict Seddon, date: 12/20/2017, view: 9434, Q&A: 0
    This protocol was developed to generate chimeric mice in which T lymphocytes could be stratified by age on the basis of congenic marker expression. The conditioning drug busulfan is used to ablate host haematopoietic stem cells while leaving the ...
    In vitro Homeostatic Proliferation of Human CD8 T Cells
    Authors:  Hossam A. Abdelsamed, Caitlin C. Zebley and Ben Youngblood, date: 11/20/2017, view: 10449, Q&A: 0
    Long-lived T-cell–mediated immunity requires persistence of memory T cells in an antigen-free environment while also maintaining a heightened capacity to recall effector functions. Such antigen-independent homeostatic proliferation is mediated in ...
    Efficient Production of Functional Human NKT Cells from Induced Pluripotent Stem Cells − Reprogramming of Human Vα24+iNKT Cells
    Authors:  Daisuke Yamada, Tomonori Iyoda, Kanako Shimizu, Yusuke Sato, Haruhiko Koseki and Shin-ichiro Fujii, date: 05/20/2017, view: 11325, Q&A: 0
    Antigen-specific T cell-derived induced pluripotent stem cells (iPSCs) have been shown to re-differentiate into functional T cells and thus provide a potential source of T cells that could be useful for cancer immunotherapy. Human Vα24+ ...



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