Developmental Biology


    Preparation of Cardiac Extracts from Embryonal Hearts to Capture RNA–protein Interactions by CLIP

    The interaction of RNA with specific RNA-binding proteins (RBP) leads to the establishment of complex regulatory networks through which gene expression is controlled. Careful consideration should be given to the exact environment where a given

    Isolation of Embryonic Cardiomyocytes and Cell Proliferation Assay Using Genetically Engineered Reporter Mouse Model
    Authors:  Maren Beall, Deqiang Li and Jihyun Jang, date: 09/05/2023, view: 458, Q&A: 0

    Congenital heart disease (CHD) is often associated with myogenic defects. During heart development, cardiomyocyte growth requires essential cues from extrinsic factors such as insulin-like growth factor 2 (IGF-2). To determine whether and how growth

    Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells
    Authors:  Fatma Uzbas and Adam C. O’Neill, date: 09/05/2023, view: 429, Q&A: 0

    The centrosome governs many pan-cellular processes including cell division, migration, and cilium formation. However, very little is known about its cell type-specific protein composition and the sub-organellar domains where these protein

    Fabrication of Microfluidic Devices for Continuously Monitoring Yeast Aging
    Authors:  Richard O’Laughlin, Emerald Forrest, Jeff Hasty and Nan Hao, date: 08/05/2023, view: 264, Q&A: 0

    For several decades, aging in Saccharomyces cerevisiae has been studied in hopes of understanding its causes and identifying conserved pathways that also drive aging in multicellular eukaryotes. While the short lifespan and unicellular nature of

    Development of a Mouse Model of Hematopoietic Loss of Y Chromosome
    Authors:  Soichi Sano and Kenneth Walsh, date: 08/05/2023, view: 278, Q&A: 0

    This protocol describes the generation of chimeric mice in which the Y chromosome is deleted from a proportion of blood cells. This model recapitulates the phenomenon of hematopoietic mosaic loss of Y chromosome (mLOY), which is frequently observed

    In vivo Electroporation of Skeletal Muscle Fibers in Mice
    Authors:  Steven J. Foltz, H. Criss Hartzell and Hyojung J. Choo, date: 07/05/2023, view: 291, Q&A: 0

    In vitro models are essential for investigating the molecular, biochemical, and cell-biological aspects of skeletal muscle. Still, models that utilize cell lines or embryonic cells do not fully recapitulate mature muscle fibers in vivo. Protein

    Long-term in toto Imaging of Cellular Behavior during Nerve Injury and Regeneration
    Authors:  Weili Tian, Andrés González-Suarez and Hernán López-Schier, date: 05/05/2023, view: 464, Q&A: 0

    Accidental wounding of the peripheral nervous system leads to acute neural dysfunction. Normally, chronic deficits are overcome because peripheral nerves naturally regenerate. However, various genetic and metabolic defects can impair their natural

    Dual-Color Live Imaging of Adult Muscle Stem Cells in the Embryonic Tissues of Drosophila melanogaster
    Authors:  Monika Zmojdzian, Binoj Dhanarajan, Krzysztof Jagla and Rajaguru Aradhya, date: 02/05/2023, view: 479, Q&A: 0

    Adult muscle stem cells (MuSCs) show remarkable capability in repairing injured tissues. Studying MuSCs in suitable model organisms, which show strong homology with vertebrate counterparts, helps in dissecting the mechanisms regulating their

    A Reliable and Consistent Method to Quantify Percent Lethality and Life Span in Drosophila melanogaster
    Authors:  Priyanka Kumari, Ushashi Ain and Hena Firdaus, date: 01/20/2023, view: 687, Q&A: 0

    Drosophila melanogaster is a classic model organism to study gene function as well as toxicological effects. To study gene function, the expression of a particular gene of interest is disrupted by using the widely explorable Drosophila genetic

    Generation of iMyoblasts from Human Induced Pluripotent Stem Cells

    Skeletal muscle stem cells differentiated from human-induced pluripotent stem cells (hiPSCs) serve as a uniquely promising model system for investigating human myogenesis and disease pathogenesis, and for the development of gene editing and


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