Neuroscience

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    Colocalization Analysis for Cryosectioned and Immunostained Tissue Samples with or without Label Retention Expansion Microscopy (LR-ExM) by JACoP
    Authors:  Xiang Zhao, Jason Garcia, Loic A. Royer and Su Guo, date: 03/05/2022, view: 2213, Q&A: 0

    Asymmetric cell division (ACD) is fundamental for balancing cell proliferation and differentiation in metazoans. During active neurogenesis in the developing zebrafish forebrain, radial glia progenitors (RGPs) mainly undergo ACD to produce one

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    Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae
    Authors:  E. Anne Martin, Sundas Ijaz, Alberto E. Pereda and Adam C. Miller, date: 01/20/2022, view: 1252, Q&A: 0

    Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network

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    Enrichment of Vascular Fragments from Mouse Embryonic Brains for Endothelial Cell Analysis
    Authors:  Nicolas Santander and Thomas D. Arnold, date: 06/20/2021, view: 1856, Q&A: 0

    Endothelial cells in the brain interact with other cell types, forming the blood-brain barrier. This barrier controls the movement of solutes into and out of the brain, regulating pathophysiological processes and drug delivery to the brain. Common

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    Ex vivo Tissue Culture Protocols for Studying the Developing Neocortex
    Authors:  Takashi Namba, Christiane Haffner and Wieland B. Huttner, date: 05/20/2021, view: 2908, Q&A: 0

    The size of the neocortex and its morphology are highly divergent across mammalian species. Several approaches have been utilized for the analysis of neocortical development and comparison among different species. In the present protocol (Note: This

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    Development of a Chemical Reproductive Aging Model in Female Rats

    Women are born with an abundant but finite pool of ovarian follicles, which naturally and progressively decreased during their reproductive years until menstrual periods stop permanently (menopause). Perimenopause represents the transition from

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    Ligand and Carbohydrate Engagement (LACE) Assay and Fluorescence Quantification on Murine Neural Tissue
    Authors:  James M. Clegg and Thomas Pratt, date: 03/20/2021, view: 3290, Q&A: 0

    The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and

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    Optimized Immunostaining of Embryonic and Early Postnatal Mouse Brain Sections
    Authors:  Kawssar Harb, Michele Bertacchi and Michèle Studer, date: 01/05/2021, view: 2937, Q&A: 0

    The mammalian neocortex, the outer layer of the cerebrum and most recently evolved brain region, is characterized by its unique areal and laminar organization. Distinct cortical layers and areas can be identified by the protein expression of graded

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    A Parkinson’s Disease-relevant Mitochondrial and Neuronal Morphology High-throughput Screening Assay in LUHMES Cells

    Parkinson’s disease is a devastating neurodegenerative disorder affecting 2-3% of the population over 65 years of age. There is currently no disease-modifying treatment. One of the predominant pathological features of Parkinson’s disease

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    Whole-mount Immunohistochemistry of Adult Zebrafish Retina for Advanced Imaging
    Authors:  Mikiko Nagashima and Peter F. Hitchcock, date: 12/20/2020, view: 2730, Q&A: 0

    Immunohistochemistry is a widely used technique to examine the expression and subcellular localization of proteins. This technique relies on the specificity of antibodies and requires adequate penetration of antibodies into tissues. The latter is

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    Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter
    Authors:  Matthew Swire and Charles ffrench-Constant, date: 10/20/2020, view: 2645, Q&A: 0
    Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol ...



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