Microinjection of β-glucan Into Larval Zebrafish (Danio rerio) for the Assessment of a Trained-Like Immunity Phenotype
    Authors:  Hannah Darroch, Jonathan W. Astin and Christopher J. Hall, date: 12/05/2023, view: 32, Q&A: 0

    The innate immune system can remember previous inflammatory insults, enabling long-term heightened responsiveness to secondary immune challenges in a process termed “trained immunity.” Trained innate immune cells undergo metabolic and

    Epicutaneous Application of Mannan Induces Psoriasis-like Inflammation in an Inbred Mouse Strain
    Authors:  Huimei Wu and Kutty Selva Nandakumar, date: 10/20/2023, view: 265, Q&A: 0

    Mannan from yeast induces psoriasis-like inflammation in the skin of inbred mouse strains. Limitations of available models led us to develop a new psoriasis model with a rapid disease onset, severe disease course, short duration, and a simple and

    Monitoring Group 2 Innate Lymphoid Cell Biology in Models of Lung Inflammation
    Authors:  Jana H. Badrani, Allyssa N. Strohm, Yung-An Huang and Taylor A. Doherty, date: 07/20/2023, view: 453, Q&A: 0

    Innate lymphoid cells (ILCs) are a rare cell population subdivided into ILC1s, ILC2s, and ILC3s, based on transcription factor expression and cytokine production. In models of lung inflammation, the release of alarmins from the epithelium activates

    HDR-based CRISPR/Cas9-mediated Knockout of PD-L1 in C57BL/6 Mice

    The immune-inhibitory molecule programmed cell death ligand 1 (PD-L1) has been shown to play a role in pathologies such as autoimmunity, infections, and cancer. The expression of PD-L1 not only on cancer cells but also on non-transformed host cells

    Anti-tumor Efficacy of CD19 CAR-T in a Raji B Cell Xenografted Mouse Model
    Authors:  Qian Xiao and Xiaolei Su, date: 04/20/2023, view: 961, Q&A: 0

    Chimeric antigen receptor (CAR)-T therapy launched a new era for cancer treatments, displaying outstanding effectiveness in relapsed or refractory B-cell malignancies. Demonstrating the tumor-killing ability of CAR-Ts in mouse xenograft models

    Isolation of RNA from the Murine Colonic Tissue and qRT-PCR for Inflammatory Cytokines
    Authors:  Ibrahim M. Sayed, Kaili Inouye, Soumita Das and Laura Crotty Alexander, date: 03/20/2023, view: 1218, Q&A: 0

    E-cigarette (E-cig) inhalation affects health status by modulating inflammation profiles in several organs, including the brain, lung, heart, and colon. The effect of flavored fourth-generation pod-based E-cigs (JUUL) on murine gut inflammation ...

    A Tumor-admixture Model to Interrogate Immune Cell–dependent Tumorigenesis

    A rigorous determination of effector contributions of tumor-infiltrating immune cells is critical for identifying targetable molecular mechanisms for the development of novel cancer immunotherapies. A tumor/immune cell–admixture model is an

    Murine Double Hit Model for Neonatal Cardiopulmonary Diseases: Bronchopulmonary Dysplasia (BPD) and Pulmonary Hypertension Associated with BPD
    Authors:  Steven P. Garrick, Philip J. Berger, Marcel F. Nold and Claudia A. Nold-Petry, date: 11/05/2022, view: 811, Q&A: 0

    Bronchopulmonary dysplasia (BPD) and pulmonary hypertension associated with BPD (BPD-PH) are of multifactorial origin and share common risk factors. Most murine models of BPD expose newborn pups to only one of these risk factors—more commonly

    Combination of Sterile Injury and Microbial Contamination to Model Post-surgical Peritoneal Adhesions in Mice
    Authors:  Julia Bayer, Deborah Stroka, Paul Kubes, Daniel Candinas and Joel Zindel, date: 08/20/2022, view: 1445, Q&A: 0

    Abdominal surgeries are frequently associated with the development of post-surgical adhesions. These are irreversible fibrotic scar bands that appear between abdominal organs and the abdominal wall. Patients suffering from adhesions are at risk of

    Cost Effective Method for gDNA Isolation from the Cecal Content and High Yield Procedure for RNA Isolation from the Colonic Tissue of Mice
    Authors:  Sohini Mukhopadhyay and Palok Aich, date: 08/05/2022, view: 846, Q&A: 0

    Microbiome studies are quickly gaining momentum. Since most of the resident microbes (consisting of bacteria, fungi, and viruses) are difficult to culture, sequencing the microbial genome is the method of choice to characterize them. It is therefore


    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.