Biological Sciences


    Multiphoton Microscopy of FITC-labelled Fusobacterium nucleatum in a Mouse in vivo Model of Breast Cancer

    Over the past decades, the main techniques used to visualize bacteria in tissue have improved but are still mainly based on indirect recognition of bacteria. Both microscopy and molecular recognition are being improved, but most procedures for ...

    Comprehensive Analyses of Muscle Function, Lean and Muscle Mass, and Myofiber Typing in Mice

    Skeletal muscle disorders commonly affect the function and integrity of muscles. Novel interventions bring new potential to rescue or alleviate the symptoms associated with these disorders. In vivo and in vitro testing in mouse models allows

    Imaging of Chloroplast Movement Responses to Light Stimulation in Different Intensities in Rice
    Authors:  Hui Wu, Ziyi Wang, Yanchun Yu and Yanli Zhang, date: 02/20/2023, view: 214, Q&A: 0

    Chloroplast movement has been observed and analyzed since the 19th century. Subsequently, the phenomenon is widely observed in various plant species such as fern, moss, Marchantia polymorpha, and Arabidopsis. However, chloroplast movement in rice is

    Protocol for the Splinted, Human-like Excisional Wound Model in Mice

    While wound healing in humans occurs primarily through re-epithelization, in rodents it also occurs through contraction of the panniculus carnosus, an underlying muscle layer that humans do not possess. Murine experimental models are by far the most

    Establishment of Restraint Stress–induced Anorexia and Social Isolation–induced Anorexia Mouse Models

    Anorexia nervosa (AN) is a devastating neuropsychiatric disease with a prevalence rate of approximately 0.3%–1% among women and morbidity and mortality rates among the highest of all neuropsychiatric disorders. The disease etiology is complex

    Evaluating Plant Drought Resistance with a Raspberry Pi and Time-lapse Photography
    Authors:  Daniel N. Ginzburg and Seung Y. Rhee, date: 01/20/2023, view: 909, Q&A: 0

    Identifying genetic variations or treatments that confer greater resistance to drought is paramount to ensuring sustainable crop productivity. Accurate and reproducible measurement of drought stress symptoms can be achieved via automated,

    Parasitoid Wasp Culturing and Assay to Study Parasitoid-induced Reproductive Modifications in Drosophila
    Authors:  Madhumala K. Sadanandappa, Shivaprasad H. Sathyanarayana and Giovanni Bosco, date: 01/05/2023, view: 225, Q&A: 0

    In nature, parasitoid wasp infections are a major cause of insect mortality. Parasitoid wasps attack a vast range of insect species to lay their eggs. As a defense, insects evolved survival strategies to protect themselves from parasitoid

    PERK Pathway Inhibitors Cure Group A Streptococcal Necrotizing Fasciitis in a Murine Model

    Group A streptococcus (GAS) is a Gram-positive human pathogen that causes invasive infections with mild to life-threatening severity, like toxic shock syndrome, rheumatic heart disease, and necrotizing fasciitis (NF). NF is characterized by a

    Infection of the Developing Central Nervous System of Drosophila by Mammalian Eukaryotic and Prokaryotic Pathogens
    Authors:  Billel Benmimoun, Bente Winkler and Pauline Spéder, date: 12/05/2022, view: 313, Q&A: 0

    Pathogen invasion of the central nervous system (CNS) is an important cause of infection-related mortality worldwide and can lead to severe neurological sequelae. To gain access to the CNS cells, pathogens have to overcome the blood–brain

    Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring
    Authors:  Paula Mondragon, Sungmin Hwang, Amy Schmid and Julie A. Maupin-Furlow, date: 11/20/2022, view: 455, Q&A: 0

    The study of haloarchaea provides an opportunity to expand understanding of the mechanisms used by extremophiles to thrive in and respond to harsh environments, including hypersaline and oxidative stress conditions. A common strategy used to


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