Environmental science


    Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring
    Authors:  Paula Mondragon, Sungmin Hwang, Amy Schmid and Julie A. Maupin-Furlow, date: 11/20/2022, view: 455, Q&A: 0

    The study of haloarchaea provides an opportunity to expand understanding of the mechanisms used by extremophiles to thrive in and respond to harsh environments, including hypersaline and oxidative stress conditions. A common strategy used to

    A Semi-quantitative Scoring System for Green Histopathological Evaluation of Large Animal Models of Acute Lung Injury

    Acute respiratory distress syndrome (ARDS) is a life-threatening, high mortality pulmonary condition characterized by acute lung injury (ALI) resulting in diffuse alveolar damage. Despite progress regarding the understanding of ARDS pathophysiology,

    A Microfluidic Platform for Tracking Individual Cell Dynamics during an Unperturbed Nutrients Exhaustion
    Authors:  Théo Aspert, Basile Jacquel and Gilles Charvin, date: 07/20/2022, view: 1370, Q&A: 0

    Microorganisms have evolved adaptive strategies to respond to the autonomous degradation of their environment. Indeed, a growing culture progressively exhausts nutrients from its media and modifies its composition. Yet, how single cells react to

    A Quick Method to Quantify Iron in Arabidopsis Seedlings
    Authors:  Chandan Kumar Gautam, Huei-Hsuan Tsai and Wolfgang Schmidt, date: 03/05/2022, view: 2010, Q&A: 0

    Iron (Fe) is an indispensable micronutrient for plant growth and development. Since both deficiency, as well as a surplus of Fe, can be detrimental to plant health, plants need to constantly tune uptake rates to maintain an optimum level of Fe.

    Preference Test of Plutella xylostella Larvae upon DMNT Treatment
    Authors:  Chen Chen, Zhen Tao, Chuanhong Wang, Tengyue Wang, Hongyi Chen, Taoshan Jiang and Peijin Li, date: 11/05/2021, view: 1276, Q&A: 0

    We describe a method to test the preference of insects in response to (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). We use a device that includes a horizontal glass tube, two grooves (with activated carbon), air flow, rubber stoppers/tubes, transparent

    A Nucleocapsid-based Transcomplementation Cell Culture System of SARS-CoV-2 to Recapitulate the Complete Viral Life Cycle
    Authors:  Yanying Yu, Xiaohui Ju and Qiang Ding, date: 11/05/2021, view: 2041, Q&A: 0

    The ongoing COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). As this virus is classified as a biosafety level-3 (BSL-3) agent, the development of countermeasures and basic research methods is logistically

    Dark Respiration Measurement from Arabidopsis Shoots
    Authors:  Jose P. Fonseca, Marcus Griffiths, Larry M. York and Kirankumar S. Mysore, date: 10/05/2021, view: 2323, Q&A: 0

    Dark respiration refers to experimental measures of leaf respiration in the absence of light, done to distinguish it from the photorespiration that occurs during photosynthesis. Dark aerobic respiration reactions occur solely in the mitochondria and

    Glucose Starvation, Magnesium Ion Starvation, and Bile Stress Assays
    Authors:  Aryashree Arunima and Mrutyunjay Suar, date: 09/20/2021, view: 1505, Q&A: 0

    Salmonella enterica serovar Enteritidis (S. Enteritidis) is a leading causative pathogen for food-borne gastroenteritis. During its course of infection, it confronts myriads of physiological barriers inside the host, such as nutrient deprivation,

    Agrobacterium-mediated Transformation of Japonica Rice Using Mature Embryos and Regenerated Transgenic Plants
    Authors:  Ammar Elakhdar, Masako Fukuda and Takahiko Kubo, date: 09/20/2021, view: 2940, Q&A: 0

    Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice.

    A Standard Operative Procedure for Safe-handling of Remains and Wastes of COVID-19 Patients
    The ongoing coronavirus disease-2019 (COVID-19) pandemic has raised significant public health issues which need to be attended. To enable efficient handling of the situation and prevent the spread of the epidemic, healthcare professionals require ...

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