Stem Cell


    Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids
    Authors:  Man Chun Chiu, Cun Li, Yifei Yu, Xiaojuan Liu, Jingjing Huang, Zhixin Wan, Kwok Yung Yuen and Jie Zhou, date: 04/20/2023, view: 402, Q&A: 0

    A robust in vitro model of the human respiratory epithelium, including the alveolar and the airway epithelium, is essential for understanding the biology and pathology of the human respiratory system. We previously described a protocol to derive

    Determining Bone-forming Ability and Frequency of Skeletal Stem Cells by Kidney Capsule Transplantation and Limiting Dilution Assay
    Authors:  Hitoshi Uchida, Takamitsu Maruyama and Wei Hsu, date: 03/20/2023, view: 143, Q&A: 0

    Adult stem cells not only maintain tissue homeostasis but are also critical for tissue regeneration during injury. Skeletal stem cells are multipotent stem cells that can even generate bones and cartilage upon transplantation to an ectopic site. ...

    Laser Capture Microdissection (LCM) of Human Skin Sample for Spatial Proteomics Research
    Authors:  Qiyu Zhang, Huizi Gong, Jie Ma, Jun Li and Ling Leng, date: 03/05/2023, view: 369, Q&A: 0

    In mammals, the skin comprises several distinct cell populations that are organized into the following layers: epidermis (stratum corneum, stratum granulosum, stratum spinosum, and basal layer), basement membrane, dermis, and hypodermal

    Thrombopoietin-independent Megakaryocyte Differentiation of Hematopoietic Progenitor Cells from Patients with Myeloproliferative Neoplasms

    Primary hematopoietic stem and progenitor cell (HSPC)-derived megakaryocytes are a valuable tool for translational research interrogating disease pathogenesis and developing new therapeutic avenues for patients with hematologic disorders including

    Using BODIPY FL-Sphingolipid Analogs to Study Sphingolipid Metabolism in Mouse Embryonic Stem Cells
    Authors:  Wei Fan and Xiaoling Li, date: 11/20/2022, view: 603, Q&A: 0

    Sphingolipids are important structural components of cellular membranes. They also function as prominent signaling molecules to control a variety of cellular events, such as cell growth, differentiation, and apoptosis. Impaired sphingolipid

    Generation of iMyoblasts from Human Induced Pluripotent Stem Cells

    Skeletal muscle stem cells differentiated from human-induced pluripotent stem cells (hiPSCs) serve as a uniquely promising model system for investigating human myogenesis and disease pathogenesis, and for the development of gene editing and

    Isolation and ex vivo Expansion of Limbal Mesenchymal Stromal Cells
    Authors:  Naresh Polisetti, Lyne Sharaf, Thomas Reinhard and Günther Schlunck, date: 07/20/2022, view: 1023, Q&A: 0

    Limbal mesenchymal stromal cells (LMSC), a cellular component of the limbal stem cell niche, have the capability of determining the fate of limbal epithelial progenitor cells (LEPC), which are responsible for the homeostasis of corneal epithelium.

    Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
    Authors:  Vasavi Nallur Srinivasaraghavan, Faria Zafar and Birgitt Schüle, date: 07/20/2022, view: 1438, Q&A: 0

    To optimize differentiation protocols for stem cell-based in vitro modeling applications, it is essential to assess the change in gene expression during the differentiation process. This allows controlling its differentiation efficiency into the

    Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen
    Authors:  Isabelle J. Marié, Lara Brambilla and David E. Levy, date: 06/05/2022, view: 1525, Q&A: 0

    Transplantation of hematopoietic material into recipient mice is an assay routinely used to determine the presence and function of hematopoietic stem and progenitor cells (HSPCs) in vivo. The principle of the method is to transplant donor cells

    Click-iT® Plus OPP Alexa Fluor® Protein Synthesis Assay in Embryonic Cells

    This protocol describes a method to assess relative changes in the level of global protein synthesis in the preimplantation embryo using the Click-iT® Plus OPP Protein Synthesis Assays. In this assay, O-propargyl-puromycin (OPP), an analog of


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