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    Striatal Synaptosomes Preparation from Mouse Brain
    Authors:  Shiqi Ma and Alexander Sorkin, date: 09/05/2018, view: 234, Q&A: 0
    The striatum is located in the subcortical region of the forebrain, it contains medium spiny neurons, cholinergic interneurons and GABAergic interneurons, and receives dopaminergic projection in the nigrostriatal pathway. This protocol provides a ...
    Combinations of Patch-Clamp and Confocal Calcium Imaging in Acutely Isolated Adult Mouse Amygdala Brain Slices
    Authors:  Jianyang Du and Erin Elizabeth Koffman, date: 08/05/2018, view: 460, Q&A: 0
    Calcium imaging is a powerful technique in the study of neuronal physiology, as it avoids the enzyme treatment on neurons and is able to study the neuronal activities in vivo. Using calcium-imaging techniques, we are able to monitor the ...
    Morphological Analysis of Dopaminergic Neurons with Age Using Caenorhabditis elegans GFP Reporter Strains
    Authors:  Sanjib Guha, Guy Caldwell and Pankaj Kapahi, date: 05/05/2018, view: 1001, Q&A: 0
    This protocol describes how to quantify different morphological defects as observed in the dopaminergic neurons using C. elegans GFP reporter strains with age.
    Flourescent Immunostaining Protocol for a-Bungorotoxin (AChRs) in Zebrafish
    Author:  Lili Jing, date: 02/20/2012, view: 9496, Q&A: 0
    Zebrafish embryo is convenient for studying neuromuscular system due to its transparency. This protocol is a rapid method to visualize the AChR clusters on the postsynaptic muscle membrane.
    Superoxide Measurement
    Author:  Hui-Ming Gao, date: 11/20/2011, view: 8510, Q&A: 1
    Superoxide dismutase (SOD)-inhibitable reduction of tetrazolium salt, WST-1, is used to measure extracellular superoxide free radical release from various immune cells such as macrophage, neutrophils, microglia (brain macrophage) after stimulation. ...
    Stereotaxic Injection of LPS or Your Reagents of Choice into Rat Substantia Nigra
    Author:  Hui-Ming Gao, date: 11/20/2011, view: 7701, Q&A: 0
    Stereotaxic injection is an invaluable tool for the creation of site-targeted lesions, injection of anatomical tracers, gene delivery by recombinant adeno-associated viruses and lentiviruses in mice and rats. Stereotaxic injection of LPS or ...
    Generation of Neuron-enriched Cultures (Method 1)
    Author:  Huiming Gao, date: 11/05/2011, view: 7837, Q&A: 0
    This protocol will guide you through the process for generating midbrain neuronal cultures from late embryo mouse brain. These cultures serve as a useful tool to study molecular pathways during neuronal death in various neurological disorders. This ...
    Generation of Neuron-enriched Cultures (Method 2)
    Author:  Huiming Gao, date: 11/05/2011, view: 8969, Q&A: 0
    Neuron-enriched cultures are a useful tool to study neuronal development and the molecular pathways that come in to play during neuronal death in various neurological disorders. This protocol is for generating midbrain neuronal cultures from late ...
    Generation of Microglia Cultures and Mixed Glial Culture
    Author:  Huiming Gao, date: 11/05/2011, view: 14439, Q&A: 2
    Primary rodent microglia-enriched cultures are the most popular model to study microglial biology in vitro and to explore immune signaling pathways. Mixed glial cultures that contain microglia and astroglia are very useful for investigating ...