Cell Biology

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    Immunophenotyping and Intracellular Staining of Fixed Whole Blood for Mass Cytometry (CyTOF)
    Authors:  Sangeeta Kowli and Holden Maecker, date: 09/05/2020, view: 649, Q&A: 0
    In this report, we present the implementation of mass cytometry for intracellular staining using fixed whole blood. In our assay described here, 250 µl of whole blood, is stimulated in vitro with PMA/ionomycin (or left unstimulated), in the ...
    A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays
    Authors:  Michael D. Caponegro, Kaitlyn Koenig Thompson, Maryam Tayyab and Stella E. Tsirka, date: 08/05/2020, view: 426, Q&A: 0
    Studying monocytic cells in isolated systems in vitro contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as ...
    Preparing Immunolocalization Slides of Maize Meiotic Chromosomes for Three-dimensional Microscopy
    Authors:  Qian Du and Wojciech P. Pawlowski, date: 07/20/2020, view: 315, Q&A: 0
    The protocol provides fully detailed steps for preparing microscopic slides of acrylamide-embedded maize meiotic cells. This method is particularly useful for examining chromatin structure and chromosome arrangement without destroying the ...
    Toluidine Blue O Staining of Paraffin-Sectioned Maize Tissue
    Authors:  Josh Strable, Jeffrey R. Yen, Michael J. Scanlon and Anne W. Sylvester, date: 05/05/2020, view: 645, Q&A: 0
    Histology incorporates microtechnique, the preparation of living material for observation and microscopy to study the anatomy of cells, tissues and organs. Interpreting histological patterns in a specimen may help to correlate form and function at ...
    Chiasma Counting in Maize Male Meiocytes
    Authors:  Gaganpreet K. Sidhu and Wojciech P. Pawlowski, date: 05/05/2020, view: 754, Q&A: 0
    This protocol combines a classical chromosome spreading technique with 3-dimensional image reconstruction for visualization of bivalent configurations during meiosis to aid chiasma counting.
    Immunolabeling of Maize Meiocytes
    Authors:  Mateusz Zelkowski and Wojciech P. Pawlowski, date: 04/05/2020, view: 851, Q&A: 0
    This protocol describes a quick method for immunolocalization of meiotic proteins in maize. It includes a fixation step that allows for long-term storage of material and provides good preservation of chromatin structure.
    Transient Transformation of Maize BMS Suspension Cells via Particle Bombardment
    Authors:  Liang-Zi Zhou and Thomas Dresselhaus, date: 12/05/2019, view: 1123, Q&A: 0
    Maize is one of the most important crop species and serves also as a model plant for grass research. A major bottleneck in maize research is stable transformation, which is both time and cost consuming, but also a technical challenge for most labs ...
    Cellular Retention Assay to Determine the Interaction Frequency of CD19-directed Chimeric Antigen Receptor (CAR) Engineered Cells against CD19+ Leukemic Cells
    Authors:  Marvin I. De los Santos and Samuel D. Bernal, date: 09/05/2019, view: 1539, Q&A: 0
    Cancer recognition by chimeric antigen receptor T (CAR-T) cell is prerequisite for cancer killing to occur. Anomalies in the binding specificity of scFv in the CAR construct may prevent successful eradication of these cancer cells. In some cases, ...
    Isolation and Efficient Maize Protoplast Transformation
    Authors:  Lina Gomez-Cano, Fan Yang and Erich Grotewold, date: 08/20/2019, view: 2257, Q&A: 0
    Establishing plant gene function and the study of gene expression regulation often require that genes (or gene variants) are introduced into plants and their activity somehow assayed. Some plants (e.g., Arabidopsis) are easily ...
    Double Labeling of Microtubules and Actin Filaments in Maize Leaf Division Zone
    Authors:  Qiong Nan, Janette Mendoza and Michelle Renee Facette, date: 06/20/2019, view: 1833, Q&A: 0
    Maize leaves grow in a gradient. The base of young maize leaves contains a high proportion of symmetrically and asymmetrically dividing cells, making the leaf an effective model system for the study of cell division. Here, we describe a double ...



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