Cell Biology

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Western Blot in Maize

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    Staining Maize Epidermal Leaf Peels with Toluidine Blue O
    Authors:  Marschal A. Bellinger, Sukhmani K. Sidhu and Carolyn G. Rasmussen, date: 04/20/2019, view: 317, Q&A: 0
    Maize is an important model organism for understanding cell patterning and development. The regular patterning of maize leaf epidermal cells has previously been characterized using the dye toluidine blue O (TBO). The polychromatic dye TBO ...
    Glue Impressions of Maize Leaves and Their Use in Classifying Mutants
    Authors:  Lindy A. Allsman, Raquel N. Dieffenbacher and Carolyn G. Rasmussen, date: 04/05/2019, view: 659, Q&A: 0
    Maize cells are surrounded by cell walls that can be easily visualized using a simple protocol outlined below. A glue impression is an imprint of maize epidermal cells on a glass slide made using a cyanoacrylate-based glue (super glue) that produces ...
    Mating and Tetrad Dissection in Chlamydomonas
    Author:  Frej Tulin, date: 04/05/2019, view: 364, Q&A: 0
    The ability to carry out controlled genetic crosses has contributed to making the unicellular green alga Chlamydomonas reinhardtii an important model organism for the green plant kingdom (Merchant et al., 2007). This protocol ...
    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 1058, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    Immunohistochemistry Staining for Tumor-associated Macrophage Polarization in Murine Subcutaneous Colon Tumor Allografts
    Authors:  Gage J. Greening, Shelby N. Bess and Timothy J. Muldoon, date: 12/05/2018, view: 1492, Q&A: 0
    Tumor-associated macrophages are the most abundant immune cells in the tumor microenvironment and may express both anti-tumor and pro-tumor functions depending on their polarization towards an M1 (classical) or M2 (alternative) phenotype, ...
    Generation of Endothelial Cells from Human Induced Pluripotent Stem Cells
    Authors:  Chun Liu, Linling Cheng, Christopher Li Chen and Nazish Sayed, date: 11/20/2018, view: 1183, Q&A: 4
    Endothelial cells (ECs), as important constituent of blood vessel are crucial to maintaining vascular tone, and any dysfunction in ECs could lead to many diseases of the vascular system. Due to limited availability of human samples, most of our ...
    A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
    Authors:  Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2018, view: 848, Q&A: 0
    Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...
    Dissection and Whole Mount Staining of Retina from Neonatal Mice
    Authors:  Mitsutaka Ogawa and Tetsuya Okajima, date: 10/05/2018, view: 1367, Q&A: 0
    Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice (Sawaguchi et al., 2017) by modifying the original protocols (Powner et al., 2012; ...
    Striatal Synaptosomes Preparation from Mouse Brain
    Authors:  Shiqi Ma and Alexander Sorkin, date: 09/05/2018, view: 895, Q&A: 0
    The striatum is located in the subcortical region of the forebrain, it contains medium spiny neurons, cholinergic interneurons and GABAergic interneurons, and receives dopaminergic projection in the nigrostriatal pathway. This protocol provides a ...
    Ty1 Retrotransposition Frequency Assay Using a Chromosomal Ty1his3AI or Ty1kanMXAI Element
    Author:  M. Joan Curcio, date: 09/05/2018, view: 863, Q&A: 0
    Here I describe a simple genetic assay to determine the frequency of retrotransposition of a single chromosomal Ty1 element that is marked with the retrotransposition indicator gene, his3AI or kanMXAI. The assay is used to ...