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    Morphological Analysis of Dopaminergic Neurons with Age Using Caenorhabditis elegans GFP Reporter Strains
    Authors:  Sanjib Guha, Guy Caldwell and Pankaj Kapahi, date: 05/05/2018, view: 161, Q&A: 0
    This protocol describes how to quantify different morphological defects as observed in the dopaminergic neurons using C. elegans GFP reporter strains with age.
    Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
    Authors:  Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 201, Q&A: 0
    The TaqMan Gene Expression Cells-to-CtTM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-105 cells, though the ...
    RNA Extraction from Wistar Rat Cochlea for qRT-PCR
    Authors:  Janaína Cândida Rodrigues and Rubens Vuono de Brito Neto, date: 12/05/2017, view: 767, Q&A: 0
    Otology research has developed considerably in recent years and molecular analysis is crucial to identify metabolic pathways and therapeutic targets. However, the structure of the cochlea limits the amount of cell mass, and special care is required ...
    MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
    Authors:  Snehalata Majumdar, Titir Guha and Rita Kundu, date: 11/20/2017, view: 1075, Q&A: 0
    Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this ...
    A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
    Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 1431, Q&A: 0
    This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
    Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface
    Author:  Nidhi Sharma, date: 09/05/2017, view: 2040, Q&A: 0
    In this protocol, leaves are cleared and fixed in an ethanol and acetic acid solution, and mounted in Hoyer’s solution. The cleared leaves are imaged under differential interference contrast (DIC) microscope. This protocol is beneficial for studying ...
    Oil Red O Staining of Fixed Worm
    Author:  Fanglian He, date: 07/05/2012, view: 15391, Q&A: 1
    Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco.
    Immunofluorescence Detection and F-actin Staining of MTLn3 Cells
    Author:  Huan Pang, date: 04/20/2012, view: 9935, Q&A: 0
    Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...
    Isolation of Endothelial Cells from Mice
    Author:  Huan Pang, date: 04/20/2012, view: 14234, Q&A: 3
    Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. In this protocol, attaining and maintaining vitality of the organ is critical. Minimizing the processing time between removing the organ and beginning ...
    Make Human Umbilical Vein Endothelial Cells from Cords
    Author:  Huan Pang, date: 04/20/2012, view: 8053, Q&A: 0
    Human umbilical vein endothelial cells (HUVEC) can be isolated from normal human umbilical veins, and are responsive to cytokine stimulation by expressing cell adhesion molecules. These cell systems are commonly used for physiological and ...