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    MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
    Authors:  Snehalata Majumdar, Titir Guha and Rita Kundu, date: 11/20/2017, view: 637, Q&A: 0
    Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this ...
    Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface
    Author:  Nidhi Sharma, date: 09/05/2017, view: 1361, Q&A: 0
    In this protocol, leaves are cleared and fixed in an ethanol and acetic acid solution, and mounted in Hoyer’s solution. The cleared leaves are imaged under differential interference contrast (DIC) microscope. This protocol is beneficial for studying ...
    Extraction of Root Apoplastic Wall Fluid for Apoplastic Peroxidase Activity Assay
    Author:  Xiyan Li, date: 09/05/2011, view: 9565, Q&A: 0
    Plant roots secret a lot of peroxidases to counteract environmental influences. This protocol describes a way to extract root apoplastic wall fluid from Arabidopsis plants and to determine the peroxidase activity using guaiacol as substrate.
    Arabidopsis Growing Protocol – A General Guide
    Author:  Xiyan Li, date: 09/05/2011, view: 17157, Q&A: 1
    Arabidopsis as the model organism for higher plants is widely studied among plant biology labs around the world. However, taking care of this tiny plant may not be trivial. Here is a general guide used for the Heven Sze lab at the University of ...
    Infiltration of Nicotiana benthamiana Protocol for Transient Expression via Agrobacterium
    Author:  Xiyan Li, date: 07/20/2011, view: 37579, Q&A: 1
    Transient expression in tobacco plant (Nicotiana benthamiana) is used to determine the subcellular location of a protein of interest when tagged with a reporter such as green fluorescent protein (GFP), or to mass produce proteins without ...
    Extract Genomic DNA from Arabidopsis Leaves (Can be Used for Other Tissues as Well)
    Author:  Yongxian Lu, date: 07/05/2011, view: 18236, Q&A: 1
    This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.
    Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis
    Author:  Xiyan Li, date: 07/05/2011, view: 18378, Q&A: 3
    Promoter-driven GUS (beta-glucuronidase) activity is the most commonly used technique for tissue-specific expression patterns in Arabidopsis. In this procedure, GUS enzyme converts 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc) to a blue product. ...
    DNA Extraction from Dried Plant Tissues Using 96-well Format (cTab Method)
    Author:  Yongxian Lu, date: 07/05/2011, view: 11762, Q&A: 5
    This high throughput DNA isolation protocol is used to extract DNA of high quality from plant tissues for various genetics studies, like genotyping, and mapping etc. This protocol uses the well-established CTAB extraction procedure, and has been ...
    Arabidopsis Pollen Tube Aniline Blue Staining
    Author:  Yongxian Lu, date: 06/20/2011, view: 17811, Q&A: 1
    The aim of this experiment is to track pollen tube growth in vivo in the female tissues after pollination. This can be used to phenotype pollen germination, tube growth and guidance, and reception.
    Arabidopsis Stomatal Opening and Closing Experiment
    Author:  Zhixin Zhao, date: 05/20/2011, view: 11166, Q&A: 1
    Stomata pores are mainly localized in the lower epidermis of plant leaves and transpirational water loss occurs through these pores. ABA inhibits light-promoted stomatal opening and promotes stomatal closure. This protocol describes how to measure ...