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Chiasma Counting in Maize Male Meiocytes

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Optimize Highly Efficient Genetic Transformation Method of Spring Wheat (Triticum aestivum L.) Cultivars Using Mature Embryo

Authors: Dongjin Kim and Megan Hager, date: 11/20/2020, view: 102, Q&A: 0

Genetic engineering is a powerful tool to develop desired traits in crop plants to make the crops suitable for future demand and changing environments. For the genetic engineering of wheat cultivars, the development of efficient transformation and ...

A Low-volume Hydroponic Protocol for Maize and A Sensitive Bud-length Assay for Root-to-Shoot Impacts of The Strigolactone Analog, rac-GR24

Authors: Jiahn-Chou Guan and Karen E. Koch, date: 08/20/2020, view: 359, Q&A: 0

Hydroponic approaches have been a boon for root research by facilitating root-feeding studies as well as secretion analysis. Results have aided our understanding of root-shoot signaling, transport, hormone function and more. However, existing ...

Seed Sterilization and Seedling Growth on Plates in the Model Grass Brachypodium distachyon

Authors: Aylin S Haas and Michael T. Raissig, date: 08/05/2020, view: 605, Q&A: 0

Brachypodium distachyon is a model grass closely related to wheat and barley. New resources and methods are being developed and Brachypodium is becoming a popular model for developmental biology, cell wall biology, (phylo) ...

Preparing Immunolocalization Slides of Maize Meiotic Chromosomes for Three-dimensional Microscopy

Authors: Qian Du and Wojciech P. Pawlowski, date: 07/20/2020, view: 465, Q&A: 0

The protocol provides fully detailed steps for preparing microscopic slides of acrylamide-embedded maize meiotic cells. This method is particularly useful for examining chromatin structure and chromosome arrangement without destroying the ...

Barley Transformation by Particle Bombardment Using Callus

Author: Sung-Yong Kim, date: 06/05/2020, view: 760, Q&A: 0

The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to ...

Toluidine Blue O Staining of Paraffin-Sectioned Maize Tissue

Authors: Josh Strable, Jeffrey R. Yen, Michael J. Scanlon and Anne W. Sylvester, date: 05/05/2020, view: 967, Q&A: 0

Histology incorporates microtechnique, the preparation of living material for observation and microscopy to study the anatomy of cells, tissues and organs. Interpreting histological patterns in a specimen may help to correlate form and function at ...

Chiasma Counting in Maize Male Meiocytes

Authors: Gaganpreet K. Sidhu and Wojciech P. Pawlowski, date: 05/05/2020, view: 970, Q&A: 0

This protocol combines a classical chromosome spreading technique with 3-dimensional image reconstruction for visualization of bivalent configurations during meiosis to aid chiasma counting.

Immunolabeling of Maize Meiocytes

Authors: Mateusz Zelkowski and Wojciech P. Pawlowski, date: 04/05/2020, view: 1093, Q&A: 0

This protocol describes a quick method for immunolocalization of meiotic proteins in maize. It includes a fixation step that allows for long-term storage of material and provides good preservation of chromatin structure.

In vitro Conditions for Dark Growth and Analysis of Maize Seedlings

Authors: Jeffrey Farrow, Marschal A. Bellinger and Carolyn G. Rasmussen, date: 03/20/2020, view: 880, Q&A: 0

Maize is an important model organism for understanding plant traits essential for proper growth and germination. One type of growth, skotomorphogenesis, occurs in the absence of light. Seedlings grown in the absence of light exhibit dramatic ...

Using Seed Chipping to Genotype Maize Kernels

Authors: Alison M. Mills, Lindy A. Allsman, Sareen Leon and Carolyn G. Rasmussen, date: 03/20/2020, view: 1284, Q&A: 0

Zea mays (maize) is an important model organism for studying monocot growth and development. Genotyping maize in the greenhouse or field can be time consuming and costly. Here, we describe a method to remove or chip a small amount of the ...

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