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Enzymatic Assays and Enzyme Histochemistry of Tuta absoluta Feeding on Tomato Leaves

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Screening and Genetic Analysis of Ethylene-Response Mutants in Etiolated Rice Seedlings

Authors: Cui-Cui Yin, Biao Ma, He Zhao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2018, view: 234, Q&A: 0

Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ...

Growth of Chlamydomonas reinhardtii under Circadian Conditions

Authors: Volker Wagner and Maria Mittag, date: 08/20/2018, view: 473, Q&A: 0

The green biflagellate unicellular alga Chlamydomonas reinhardtii serves as a model to study fundamental biological processes such as the structure and function of flagella or light-driven processes including photosynthesis, its behavioral ...

Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers

Authors: Seijiro Ono and Ken-Ichi Nonomura, date: 08/05/2018, view: 376, Q&A: 0

This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding ...

Protocol to Test the Effect of Sorbitol in vitro on Hybrid Larch (Larix x eurolepis Henry) Emblings

Authors: Bettina Johst and María del Carmen Dacasa Rüdinger, date: 07/20/2018, view: 454, Q&A: 0

This protocol presents a method to test the effect of sorbitol in vitro on hybrid larch plants derived from somatic embryogenesis. We have tested four different media variants, one control variant without sorbitol and three variants of ...

Visualization of Growth and Morphology of Fungal Hyphae in planta Using WGA-AF488 and Propidium Iodide Co-staining

Authors: Amey Redkar, Elaine Jaeger and Gunther Doehlemann, date: 07/20/2018, view: 574, Q&A: 0

Fungal pathogens colonizing plants show a varying degree of symptoms. Microscopy techniques have been used to study the infection and proliferation of fungal hyphae inside the host. One of the best optimized and commonly used method is the ...

RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis

Authors: Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 674, Q&A: 0

RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...

Micropropagation of Prickly Pear by Axillary Shoot Proliferation

Authors: Luisa Gutiérrez-Quintana, Carlos Zúñiga-Rizo, Asdrúbal Burgos and Liberato Portillo, date: 07/05/2018, view: 536, Q&A: 0

A protocol for the axillary bud proliferation of prickly pear (Opuntia; Cactaceae) is presented. This genus is widely used as a crop in the arid and semi-arid areas of the globe worldwide, providing numerous benefits for human and animal ...

Phenol-based Extraction of RNA from Chlamydomonas reinhardtii

Authors: Emanuel Sanz-Luque and Amaury de Montaigu, date: 07/05/2018, view: 589, Q&A: 0

RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be ...

Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae

Authors: Sara Mehrabi, Suzana Stjelja and Christina Dixelius, date: 06/05/2018, view: 875, Q&A: 0

Isolation of DNA from obligate biotrophic soil-borne plant pathogens is challenging. This is because of their strict requirement of living plant tissue for their growth and propagation. A soil habitat further imposes risk of contamination from other ...

Growing and Pollinating Maize

Authors: Sarah Hake and China Lunde, date: 05/05/2018, view: 735, Q&A: 0

[Abstract] Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...