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    Ty1 Retrotransposition Frequency Assay Using a Chromosomal Ty1his3AI or Ty1kanMXAI Element
    Author:  M. Joan Curcio, date: 09/05/2018, view: 170, Q&A: 0
    Here I describe a simple genetic assay to determine the frequency of retrotransposition of a single chromosomal Ty1 element that is marked with the retrotransposition indicator gene, his3AI or kanMXAI. The assay is used to ...
    Assays for Oxidative Responses of Fusarium graminearum Strains to Superoxide Radicals
    Authors:  Yan-Zhang Wang, Yan Guo and Wei-Hua Tang, date: 09/05/2018, view: 297, Q&A: 0
    The ascomycete fungus Fusarium graminearum is a major causal agent of Fusarium head blight (FHB), a devastating disease affecting small grains cereals worldwide. To better understand the pathogenesis of this fungus, we provide here ...
    Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
    Authors:  Wan-Qiu Wang and Wei-Hua Tang, date: 08/20/2018, view: 336, Q&A: 0
    Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by ...
    Fusarium graminearum Double (Triple) Mutants Generation Using Sexual Crosses
    Authors:  Yan Guo, Dong Zhang and Wei-Hua Tang, date: 08/05/2018, view: 292, Q&A: 0
    Fusarium graminearum is a destructive phytopathogen that infects major cereal crops such as wheat, maize and barley. Double or triple mutants are often very useful in the phenotypic and genetic analysis of genes that function redundantly or ...
    Perithecium Formation and Ascospore Discharge in Fusarium graminearum
    Authors:  Yan Guo, Wan-Qian Wei, Dong Zhang and Wei-Hua Tang, date: 08/05/2018, view: 345, Q&A: 0
    The filamentous ascomycete Fusarium graminearum (previously also known as Gibberella zeae) is a phytopathogen of grain cereals, reducing crop yield and grain quality. The abilities of sexual reproduction organ-perithecium ...
    Visualization of Growth and Morphology of Fungal Hyphae in planta Using WGA-AF488 and Propidium Iodide Co-staining
    Authors:  Amey Redkar, Elaine Jaeger and Gunther Doehlemann, date: 07/20/2018, view: 574, Q&A: 0
    Fungal pathogens colonizing plants show a varying degree of symptoms. Microscopy techniques have been used to study the infection and proliferation of fungal hyphae inside the host. One of the best optimized and commonly used method is the ...
    Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
    Author:  Gal Haimovich, date: 07/20/2018, view: 562, Q&A: 0
    Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
    Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
    Authors:  Sara Mehrabi, Suzana Stjelja and Christina Dixelius, date: 06/05/2018, view: 875, Q&A: 0
    Isolation of DNA from obligate biotrophic soil-borne plant pathogens is challenging. This is because of their strict requirement of living plant tissue for their growth and propagation. A soil habitat further imposes risk of contamination from other ...
    Phenotypic Profiling of Candida glabrata in Liquid Media
    Authors:  Fabian Istel, Miha Tome, Sabrina Jenull and Karl Kuchler, date: 04/05/2017, view: 3806, Q&A: 0
    Here, we describe a method for a large-scale liquid screening approach in C. glabrata. This liquid media method offers several distinct advantages over solid media approaches. This includes growth measurement on a plate reader instead of ...
    GST-tagged Yeast Protein Purification
    Author:  Hogune Im, date: 10/05/2011, view: 11454, Q&A: 2
    Glutation S-transferase (GST) tagging is the most commonly used purification strategy for recombinant protein. It was developed with the goal of preserving the enzymatic activity by utilizing gentle elution condition of the target protein from ...