Biochemistry

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    Measurement of Secreted Embryonic Alkaline Phosphatase
    Authors:  Meiyan Wang, Xinyi Wang and Haifeng Ye, date: 02/05/2023, view: 72, Q&A: 0

    Secreted reporters have been demonstrated to be simple and useful tools for analyzing transcriptional regulation in mammalian cells. The distinctive feature of these assays is the ability to detect reporter gene expression in the culture supernatant

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    Continuous Measurement of Reactive Oxygen Species Formation in Bacteria-infected Bone Marrow–derived Macrophages Using a Fluorescence Plate Reader

    Macrophages are at the center of innate immunity and are the main target cells of the intracellular pathogen Salmonella enterica serovar Typhi. The production of reactive oxygen and nitrogen species (ROS/RNS) is the host’s early response to

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    Assay for Phytaspase-mediated Peptide Precursor Cleavage Using Synthetic Oligopeptide Substrates
    Authors:  Sven Reichardt, Annick Stintzi and Andreas Schaller, date: 02/05/2023, view: 36, Q&A: 0

    Proteases control plant growth and development by limited proteolysis of regulatory proteins at highly specific sites. This includes the processing of peptide hormone precursors to release the bioactive peptides as signaling molecules. The proteases

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    Lysate-to-grid: Rapid Isolation of Native Complexes from Budding Yeast for Cryo-EM Imaging

    Single-particle electron cryo-microscopy (cryo-EM) is an effective tool to determine high-resolution structures of macromolecular complexes. Its lower requirements for sample concentration and purity make it an accessible method to determine

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    Single Oligonucleotide Capture of RNA And Temperature Elution Series (SOCRATES) for Identification of RNA-binding Proteins
    Authors:  Allen T. Yu, Disha Aggarwal, Darryl Pappin and David L. Spector, date: 12/20/2022, view: 834, Q&A: 0

    The importance of studying the mechanistic aspects of long non-coding RNAs is being increasingly emphasized as more and more regulatory RNAs are being discovered. Non-coding RNA sequences directly associate with generic RNA-binding proteins as

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    Synthesis of Novel MicroRNA-30c Analogs to Reduce Apolipoprotein B Secretion in Human Hepatoma Cells

    Atherosclerosis, a condition characterized by thickening of the arteries due to lipid deposition, is the major contributor to and hallmark of cardiovascular disease. Although great progress has been made in lowering the lipid plaques in

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    A Direct in vitro Fatty Acylation Assay for Hedgehog Acyltransferase
    Authors:  Adina R. Schonbrun and Marilyn D. Resh, date: 12/20/2022, view: 214, Q&A: 0

    Several assays have been developed to monitor the in vitro catalytic activity of Hedgehog acyltransferase (Hhat), an enzyme critical to the Hedgehog signaling pathway in cells. However, the majority of these previously reported assays involve

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    Assessing the in vitro Binding Affinity of Protein–RNA Interactions Using an RNA Pull-down Technique
    Authors:  Anand Chopra, Feras Balbous and Kyle K. Biggar, date: 12/05/2022, view: 1174, Q&A: 0

    RNA is a vital component of the cell and is involved in a diverse range of cellular processes through a variety of functions. However, many of these functions cannot be performed without interactions with proteins. There are currently several

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    Human Auto-IgG Purification from High Volume Serum Sample by Protein G Affinity Purification
    Authors:  Serena Sensi and Andreas Goebel, date: 12/05/2022, view: 260, Q&A: 0

    Immunoglobulins are proteins produced by the immune system, which bind specifically to the antigen that induced their formation and target it for destruction. Highly purified human immunoglobulins are commonly used in research laboratories for

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