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Enzymatic Assays and Enzyme Histochemistry of Tuta absoluta Feeding on Tomato Leaves

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    Very Low Molecular Weight Proteins Electrophoresis Protocol
    Authors:  César E. Rivera, José D. Rosales, Juan C. Freites-Perez and Eliaira Rodriguez, date: 11/20/2018, view: 222, Q&A: 0
    The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...
    SDS-PAGE for Silk Fibroin Protein
    Author:  Yu-Qing Zhang, date: 10/20/2018, view: 402, Q&A: 0
    The method and detailed procedure of SDS-PAGE for silk proteins are exactly the same as for other proteins, but the electrophoresis profile of silk protein is often unsatisfactory. The main reason is that their molecular masses are too large, and ...
    A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
    Authors:  Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 886, Q&A: 0
    This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
    In vitro Chaperone Activity Assay Using α-Amylase as Target Protein
    Authors:  Jeeshma Nambidi Parambath, Gayathri Valsala, Karthik Menon and Shiburaj Sugathan, date: 06/20/2018, view: 1221, Q&A: 0
    Small heat shock proteins (sHSP) are stress proteins which are ubiquitously found in almost all living organisms. They function as molecular chaperones, which assist in protein folding during translation and in the prevention of irreversible protein ...
    Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    Authors:  Yong Joon Kim, Stefan Kaluz, Anil Mehta, Emily Weinert, Shannon Rivera and Erwin G. Van Meir, date: 09/05/2017, view: 3797, Q&A: 0
    The transcription factor Hypoxia-Inducible Factor (HIF) complexes with the coactivator p300, activating the hypoxia response pathway and allowing tumors to grow. The CH1 and CAD domains of each respective protein form the interface between p300 and ...
    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
    Author:  Zheng Liu, date: 06/20/2012, view: 8070, Q&A: 0
    Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
    RNP-IP (Original Method): Obtaining Majority RNA from RNA Binding Protein in the Nucleus
    Author:  FengZhi Liu, date: 05/20/2012, view: 15233, Q&A: 1
    Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and ...
    Antibody Affinity Purification
    Author:  Huan Pang, date: 05/05/2012, view: 9670, Q&A: 0
    Antibody purification is performed to concentrate and enrich antigen-specific antibodies and lower the background signal during detection by removing any non-specific proteins. Affinity purification makes use of specific binding interactions between ...
    Immunofluorescence Detection and F-actin Staining of MTLn3 Cells
    Author:  Huan Pang, date: 04/20/2012, view: 11383, Q&A: 0
    Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...
    PI (phosphoinositide)-3 Kinase Assay
    Author:  Huan Pang, date: 04/20/2012, view: 7858, Q&A: 1
    PI3-kinases regulate a wide range of cellular responses through the production of phosphatidylinositol 3, 4, 5-trisphosphate [PI (3, 4, 5) P (3)] in cellular membranes. This protocol provides a highly efficient and easy-to-handle method for ...




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