Developmental Biology


    E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
    Authors:  Elena Astanina, Sara Petrillo, Tullio Genova, Federico Mussano and Federico Bussolino, date: 05/05/2023, view: 172, Q&A: 0

    X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology

    Modified Pseudo-Schiff Propidium Iodide for Staining the Shoot Apical Meristem in Arabidopsis
    Authors:  Ruiqi Li, Qi Li and Ligeng Ma, date: 05/05/2023, view: 295, Q&A: 0

    Visualization of cell structure with fluorescent dye for characterizing cell size, shape, and arrangement is a common method to study tissue morphology and morphogenesis. In order to observe shoot apical meristem (SAM) in Arabidopsis thaliana by

    Assessment of Chemosensory Response to Volatile Compounds in Healthy, Aged, and Neurodegenerative Caenorhabditis elegans Models
    Authors:  Cira Crespo and Roberto Grau, date: 05/05/2023, view: 178, Q&A: 0

    A basic function of the nervous system is to confer the ability to detect external stimuli and generate appropriate behavioral and physiological responses. These can be modulated when parallel streams of information are provided to the nervous

    Long-term in toto Imaging of Cellular Behavior during Nerve Injury and Regeneration
    Authors:  Weili Tian, Andrés González-Suarez and Hernán López-Schier, date: 05/05/2023, view: 276, Q&A: 0

    Accidental wounding of the peripheral nervous system leads to acute neural dysfunction. Normally, chronic deficits are overcome because peripheral nerves naturally regenerate. However, various genetic and metabolic defects can impair their natural

    Induction of Skeletal Muscle Injury by Intramuscular Injection of Cardiotoxin in Mouse
    Authors:  Xin Fu, Sheng Li, Minzhi Jia, Wenjun Yang and Ping Hu, date: 05/05/2023, view: 275, Q&A: 0

    Skeletal muscle is the most abundant tissue in the human body and has a tremendous capability to regenerate in response to muscle injuries and diseases. Induction of acute muscle injury is a common method to study muscle regeneration in vivo.

    Analysis of Mouse Brain Sections by Live-cell Time-lapse Confocal Microscopy
    Authors:  Tao Yang, Ty Hergenreder and Bing Ye, date: 04/05/2023, view: 353, Q&A: 0

    The developing cerebral cortex of mammals is generated from nascent pyramidal neurons, which radially migrate from their birthplace in the ventral part of the neural tube to the cortical surface. Subtle aberrations in this process may cause

    Live Imaging of Phagoptosis in ex vivo Drosophila Testis
    Authors:  Diana Kanaan, Boris Shklyar, Lilach Porat-Kuperstein and Hila Toledano, date: 03/20/2023, view: 388, Q&A: 0

    Phagoptosis is a prevalent type of programmed cell death (PCD) in adult tissues in which phagocytes non-autonomously eliminate viable cells. Therefore, phagoptosis can only be studied in the context of the entire tissue that includes both the ...

    Determining Bone-forming Ability and Frequency of Skeletal Stem Cells by Kidney Capsule Transplantation and Limiting Dilution Assay
    Authors:  Hitoshi Uchida, Takamitsu Maruyama and Wei Hsu, date: 03/20/2023, view: 143, Q&A: 0

    Adult stem cells not only maintain tissue homeostasis but are also critical for tissue regeneration during injury. Skeletal stem cells are multipotent stem cells that can even generate bones and cartilage upon transplantation to an ectopic site. ...

    The Assessment of Beta Cell Mass during Gestational Life in the Mouse
    Authors:  Yury Kryvalap and Jan Czyzyk, date: 03/20/2023, view: 185, Q&A: 0

    Successful advancement in the treatment of diabetes mellitus is not possible without well-established methodology for beta cell mass calculation. Here, we offer the protocol to assess beta cell mass during embryonic development in the mouse. The ...

    Dual-Color Live Imaging of Adult Muscle Stem Cells in the Embryonic Tissues of Drosophila melanogaster
    Authors:  Monika Zmojdzian, Binoj Dhanarajan, Krzysztof Jagla and Rajaguru Aradhya, date: 02/05/2023, view: 352, Q&A: 0

    Adult muscle stem cells (MuSCs) show remarkable capability in repairing injured tissues. Studying MuSCs in suitable model organisms, which show strong homology with vertebrate counterparts, helps in dissecting the mechanisms regulating their


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