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    Make Human Umbilical Vein Endothelial Cells from Cords
    Author:  Huan Pang, date: 04/20/2012, view: 7746, Q&A: 0
    Human umbilical vein endothelial cells (HUVEC) can be isolated from normal human umbilical veins, and are responsive to cytokine stimulation by expressing cell adhesion molecules. These cell systems are commonly used for physiological and ...
    Cell Culture Transfection for Production and Purification of Wnt Ligands
    Author:  Lili Jing, date: 01/20/2012, view: 11639, Q&A: 0
    Wnt ligand proteins are extremely difficult to purify and enrich in vitro. This protocol uses Wnt11r protein as an example to illustrate how to use 293T cells to produce secreted Wnt11r and collect it in vitro for further ...
    Isolation of Primary Human Keratinocytes from Foreskin
    Author:  Jason Reuter, date: 12/20/2011, view: 9515, Q&A: 0
    Keratinocytes are the primary constituents of human skin, the functional barrier between our bodies and the external environment. The balance between keratinocyte differentiation and self-renewal is crucial to skin homeostasis. Primary keratinocyte ...
    Mouse Embryonic Stem Cell Maintenance for Differentiation
    Author:  Hogune Im, date: 10/20/2011, view: 10571, Q&A: 0
    Embryonic stem cells are derived from inner cell mass of an embryo that can differentiate into every cell type in the body. Clinically, cultured red blood cell supply is of great interest. However, some of the hurdles need to be overcome. This ...
    Culturing of C57BL/6 Mouse Embryonic Stem (ES) Cell Line
    Author:  Yuqiong Pan, date: 10/05/2011, view: 10029, Q&A: 0
    Using ATCC ES C57BL/6 as an example, it is shown here how to culture mouse embryonic stem (ES) cell line. The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008]. The ES cells ...
    In vivo BrdU Incorporation and Detection in Mouse
    Author:  Zheng Liu, date: 06/05/2011, view: 23759, Q&A: 0
    BrdU (Bromodeoxyuridine or 5-bromo-2’-deoxyuridine) is a synthetic nucleoside that is incorporated into DNA by proliferating cells. This protocol is to be used to incorporate and detect BrdU in murine plasma cells. The plasma cells described in this ...
    Making Males of C. elegans
    Author:  Fanglian He, date: 04/20/2011, view: 16533, Q&A: 2
    Getting males from a hermaphrodite population. This is a modified version of protocol originally written by Michael Koelle at Yale University.
    Lifespan Assay
    Author:  Fanglian He, date: 04/05/2011, view: 17213, Q&A: 3
    This assay is used to address aging-related questions in worms.