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Enzymatic Assays and Enzyme Histochemistry of Tuta absoluta Feeding on Tomato Leaves

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Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells

Authors: Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 1173, Q&A: 0

The TaqMan Gene Expression Cells-to-Ct^TM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-10⁵ cells, though the ...

Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example

Authors: Yong Joon Kim, Stefan Kaluz, Anil Mehta, Emily Weinert, Shannon Rivera and Erwin G. Van Meir, date: 09/05/2017, view: 3202, Q&A: 0

The transcription factor Hypoxia-Inducible Factor (HIF) complexes with the coactivator p300, activating the hypoxia response pathway and allowing tumors to grow. The CH1 and CAD domains of each respective protein form the interface between p300 and ...

Murine xenograft model

Author: FengZhi Liu, date: 06/05/2012, view: 13448, Q&A: 1

This experiment is used to test the efficacies of chemo treatments or gene therapy in an in vivo system. In this protocol, the mouse xenograft model is used.

Protocol for knockdown of HuR with siRNA

Author: FengZhi Liu, date: 05/20/2012, view: 8506, Q&A: 1

Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.

Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)

Author: Hui Zhu, date: 04/05/2012, view: 49544, Q&A: 2

Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...

Protein Extraction from Mice Xenograft Tumor

Author: Huan Pang, date: 04/05/2012, view: 15721, Q&A: 1

Proteins from frozen tissue such as xenograft tumor need to be extracted efficiently without being degraded to make the best use of a limited resource and to ensure that an accurate representation of proteins in selected tumor tissue can be ...

Protocol to Determine Mitotic Index by FACS

Author: Hui Zhu, date: 03/20/2012, view: 13278, Q&A: 0

Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...

Cell Adhesion Assay

Author: Yanling Chen, date: 03/05/2012, view: 43018, Q&A: 1

Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a ...

Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation

Author: Hui Zhu, date: 02/05/2012, view: 14137, Q&A: 0

Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively ...

Tumor Cell Invasion Assay

Author: Yanling Chen, date: 02/05/2012, view: 24708, Q&A: 0

Cell invasion assays have been used to study the interactions between tumor cells and the extracellular matrix (ECM), which not only provides a structural scaffold for the cell, but also contains various biological factors for the survival and ...