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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Cancer Biology
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Chiasma Counting in Maize Male Meiocytes
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A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays
Authors:
Michael D. Caponegro
,
Kaitlyn Koenig Thompson
,
Maryam Tayyab
and
Stella E. Tsirka
,
date:
08/05/2020,
view:
426,
Q&A:
0
Studying monocytic cells in isolated systems
in vitro
contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as ...
Cellular Retention Assay to Determine the Interaction Frequency of CD19-directed Chimeric Antigen Receptor (CAR) Engineered Cells against CD19
+
Leukemic Cells
Authors:
Marvin I. De los Santos
and
Samuel D. Bernal
,
date:
09/05/2019,
view:
1539,
Q&A:
0
Cancer recognition by chimeric antigen receptor T (CAR-T) cell is prerequisite for cancer killing to occur. Anomalies in the binding specificity of scFv in the CAR construct may prevent successful eradication of these cancer cells. In some cases, ...
Immunohistochemistry Staining for Tumor-associated Macrophage Polarization in Murine Subcutaneous Colon Tumor Allografts
Authors:
Gage J. Greening
,
Shelby N. Bess
and
Timothy J. Muldoon
,
date:
12/05/2018,
view:
5415,
Q&A:
0
Tumor-associated macrophages are the most abundant immune cells in the tumor microenvironment and may express both anti-tumor and pro-tumor functions depending on their polarization towards an M1 (classical) or M2 (alternative) phenotype, ...
Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
Authors:
Kirsten A. Copren
,
Oyang Teng
,
Cassandra J. Adams
,
Cláudia Bispo
and
Michael Lee
,
date:
04/20/2018,
view:
4902,
Q&A:
0
The TaqMan Gene Expression Cells-to-Ct
TM
Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-10
5
cells, though the ...
Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
Authors:
Yong Joon Kim
,
Stefan Kaluz
,
Anil Mehta
,
Emily Weinert
,
Shannon Rivera
and
Erwin G. Van Meir
,
date:
09/05/2017,
view:
8781,
Q&A:
0
The transcription factor Hypoxia-Inducible Factor (HIF) complexes with the coactivator p300, activating the hypoxia response pathway and allowing tumors to grow. The CH1 and CAD domains of each respective protein form the interface between p300 and ...
Murine xenograft model
Author:
FengZhi Liu
,
date:
06/05/2012,
view:
18823,
Q&A:
1
This experiment is used to test the efficacies of chemo treatments or gene therapy in an
in vivo
system. In this protocol, the mouse xenograft model is used.
Protocol for knockdown of HuR with siRNA
Author:
FengZhi Liu
,
date:
05/20/2012,
view:
10616,
Q&A:
1
Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
Author:
Hui Zhu
,
date:
04/05/2012,
view:
60681,
Q&A:
2
Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
Protein Extraction from Mice Xenograft Tumor
Author:
Huan Pang
,
date:
04/05/2012,
view:
21315,
Q&A:
1
Proteins from frozen tissue such as xenograft tumor need to be extracted efficiently without being degraded to make the best use of a limited resource and to ensure that an accurate representation of proteins in selected tumor tissue can be ...
Protocol to Determine Mitotic Index by FACS
Author:
Hui Zhu
,
date:
03/20/2012,
view:
16773,
Q&A:
0
Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...
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