Cancer Biology


    Thrombopoietin-independent Megakaryocyte Differentiation of Hematopoietic Progenitor Cells from Patients with Myeloproliferative Neoplasms

    Primary hematopoietic stem and progenitor cell (HSPC)-derived megakaryocytes are a valuable tool for translational research interrogating disease pathogenesis and developing new therapeutic avenues for patients with hematologic disorders including

    Cell-derived Matrix Assays to Assess Extracellular Matrix Architecture and Track Cell Movement

    The extracellular matrix (ECM) is a non-cellular network of macromolecules, which provides cells and tissues with structural support and biomechanical feedback to regulate cellular function, tissue tension, and homeostasis. Even subtle changes

    Arrayed CRISPR/Cas9 Screening for the Functional Validation of Cancer Genetic Dependencies

    CRISPR/Cas9 screening has revolutionized functional genomics in biomedical research and is a widely used approach for the identification of genetic dependencies in cancer cells. Here, we present an efficient and versatile protocol for the

    A Direct in vitro Fatty Acylation Assay for Hedgehog Acyltransferase
    Authors:  Adina R. Schonbrun and Marilyn D. Resh, date: 12/20/2022, view: 214, Q&A: 0

    Several assays have been developed to monitor the in vitro catalytic activity of Hedgehog acyltransferase (Hhat), an enzyme critical to the Hedgehog signaling pathway in cells. However, the majority of these previously reported assays involve

    Analysis of N6-methyladenosine RNA Modification Levels by Dot Blotting
    Authors:  Yu Du, Mingyue Xia and Zhigang Hu, date: 12/05/2022, view: 394, Q&A: 0

    N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNAs (mRNAs), affecting their fold, stability, degradation, and cellular interaction(s) and implicating them in processes such as splicing, translation,

    Fluorescence Time-lapse Imaging of Entosis Using Tetramethylrhodamine Methyl Ester Staining
    Authors:  Emir Bozkurt, Heiko Düssmann and Jochen H. M. Prehn, date: 12/05/2022, view: 326, Q&A: 0

    Entosis is a process where a living cell launches an invasion into another living cell’s cytoplasm. These inner cells can survive inside outer cells for a long period of time, can undergo cell division, or can be released. However, the fate

    OxiDIP-Seq for Genome-wide Mapping of Damaged DNA Containing 8-Oxo-2'-Deoxyguanosine
    Authors:  Francesca Gorini, Giovanni Scala, Susanna Ambrosio, Barbara Majello and Stefano Amente, date: 11/05/2022, view: 645, Q&A: 0

    8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) is considered to be a premutagenic DNA lesion generated by 2'-deoxyguanosine (dG) oxidation due to reactive oxygen species (ROS). In recent years, the 8-oxodG distribution in human, mouse, and

    An Unbiased CRISPR-Cas9 Screening Method for the Identification of Positive and Negative Regulatory Proteins of Cell Adhesion

    Mature B-cell lymphomas are highly dependent upon the protective lymphoid organ microenvironment for their growth and survival. Targeting integrin-mediated homing and retention of the malignant B cells in the lymphoid organs, using the

    Analysis of the Ubiquitination and Phosphorylation of Vangl Proteins
    Authors:  Di Feng, Ziwei He and Bo Gao, date: 10/20/2022, view: 913, Q&A: 0

    The core planar cell polarity (PCP) protein Vang/Vangl, including Vangl1 and Vangl2 in vertebrates, is indispensable during development. Our previous studies showed that the activity of Vangl is tightly controlled by two important posttranslational

    Gastrulation Screening to Identify Anti-metastasis Drugs in Zebrafish Embryos
    Authors:  Joji Nakayama, Hideki Makinoshima and Zhiyuan Gong, date: 10/05/2022, view: 674, Q&A: 0

    Few models exist that allow for rapid and effective screening of anti-metastasis drugs. Here, we present a drug screening protocol utilizing gastrulation of zebrafish embryos for identification of anti-metastasis drugs. Based on the evidence that


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