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    Protein-ligand Binding Assay by Liquid Chromatography-Mass Spectrometry
    Author:  Xiyan Li, date: 08/20/2011, view: 11440, Q&A: 0
    Protein-small molecule binding coefficients are determined by quantitative LC-MS in this method. Traditional biochemical plot is consequently used to set up the binding curve between a known protein and a small molecule (<1000 da) to determine the affinity constant (kd) and the stoichiometry (bmax). da)="" to="" determine="" the="" affinity="" constant="" (kd)="" and="" the="" stoichiometry=""> ...
    Rabbit IgG Conjugation to Dynabeads
    Author:  Xiyan Li, date: 05/05/2011, view: 10573, Q&A: 1
    This method couples rabbit IgG (or any other proteins serve as affinity reagent) to the surface of magnetic beads (Brand name: Dynabeads). The amine and thiol groups of amino acid residues on the protein are covalently linked with the epoxy group on ...
    In-Solution Digestion Of Purified Yeast Protein For LC-MS
    Author:  Xiyan Li, date: 04/20/2011, view: 9527, Q&A: 0
    This method describes the preparation of total yeast protein extract for mass spectrometry analysis. The protein extract is digested by trypsin in a solution with strong denaturants. The digested sample is dried and re-constituted in a mixture ...
    Affinity Purification of Yeast Protein-interacting Metabolites for ESI-MS Analysis
    Author:  Xiyan Li, date: 04/20/2011, view: 10501, Q&A: 5
    The method described here can be used to discover in vivo protein-metabolite interactions. Metabolite-protein complexes are purified from yeast cell lysates by an affinity tag that recognizes the protein of interest. The protein-bound metabolites ...