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    Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
    Authors:  Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 200, Q&A: 0
    The TaqMan Gene Expression Cells-to-CtTM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-105 cells, though the ...
    RNA Extraction from Wistar Rat Cochlea for qRT-PCR
    Authors:  Janaína Cândida Rodrigues and Rubens Vuono de Brito Neto, date: 12/05/2017, view: 765, Q&A: 0
    Otology research has developed considerably in recent years and molecular analysis is crucial to identify metabolic pathways and therapeutic targets. However, the structure of the cochlea limits the amount of cell mass, and special care is required ...
    Cryopreservation of Human Serum
    Authors:  Yael Rosenberg-Hasson and Holden Maecker, date: 11/05/2015, view: 2767, Q&A: 0
    This protocol describes how to collect and aliquot human serum and store at -80 °C for future cytokine/chemokine/protein profile analysis.
    Please use personal protective equipment (PPE) as required when handling samples with potential ...
    Site Density Protocol
    Author:  Jun Huang, date: 07/05/2012, view: 5003, Q&A: 0
    The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T ...
    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
    Author:  Zheng Liu, date: 06/20/2012, view: 7074, Q&A: 0
    Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
    Murine xenograft model
    Author:  FengZhi Liu, date: 06/05/2012, view: 12227, Q&A: 1
    This experiment is used to test the efficacies of chemo treatments or gene therapy in an in vivo system. In this protocol, the mouse xenograft model is used.
    RNP-IP (Original Method): Obtaining Majority RNA from RNA Binding Protein in the Nucleus
    Author:  FengZhi Liu, date: 05/20/2012, view: 13670, Q&A: 1
    Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and ...
    Protocol for knockdown of HuR with siRNA
    Author:  FengZhi Liu, date: 05/20/2012, view: 7873, Q&A: 1
    Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
    Antibody Affinity Purification
    Author:  Huan Pang, date: 05/05/2012, view: 8349, Q&A: 0
    Antibody purification is performed to concentrate and enrich antigen-specific antibodies and lower the background signal during detection by removing any non-specific proteins. Affinity purification makes use of specific binding interactions between ...
    Cell Culture Mycoplasma Detection by PCR
    Author:  Huan Pang, date: 04/20/2012, view: 9911, Q&A: 0
    DNA was extracted from the supernatant of each sample. After PCR-amplification of mycoplasma DNA, detection was performed by gel electrophresis. The PCR primers were designed to cover the consensus sequences that can detect all types of mycoplasma ...