Mammalia

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Western Blot in Maize

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    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 1058, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    Immunohistochemistry Staining for Tumor-associated Macrophage Polarization in Murine Subcutaneous Colon Tumor Allografts
    Authors:  Gage J. Greening, Shelby N. Bess and Timothy J. Muldoon, date: 12/05/2018, view: 1492, Q&A: 0
    Tumor-associated macrophages are the most abundant immune cells in the tumor microenvironment and may express both anti-tumor and pro-tumor functions depending on their polarization towards an M1 (classical) or M2 (alternative) phenotype, ...
    Generation of Endothelial Cells from Human Induced Pluripotent Stem Cells
    Authors:  Chun Liu, Linling Cheng, Christopher Li Chen and Nazish Sayed, date: 11/20/2018, view: 1183, Q&A: 4
    Endothelial cells (ECs), as important constituent of blood vessel are crucial to maintaining vascular tone, and any dysfunction in ECs could lead to many diseases of the vascular system. Due to limited availability of human samples, most of our ...
    Caesarean Sectioning and Cross-Fostering of the Mouse
    Authors:  Zheping Cai, Guohong Wang, Yang Chen and Miao He, date: 11/20/2018, view: 1098, Q&A: 0
    A caesarean section is required if the recipient of an embryo transfer or any pregnant mouse has not given birth by the delivery time that is normal for the particular strain. It is commonly used to derive a strain of pathogen-free mice from a ...
    A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
    Authors:  Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2018, view: 848, Q&A: 0
    Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...
    Dissection and Whole Mount Staining of Retina from Neonatal Mice
    Authors:  Mitsutaka Ogawa and Tetsuya Okajima, date: 10/05/2018, view: 1367, Q&A: 0
    Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice (Sawaguchi et al., 2017) by modifying the original protocols (Powner et al., 2012; ...
    Striatal Synaptosomes Preparation from Mouse Brain
    Authors:  Shiqi Ma and Alexander Sorkin, date: 09/05/2018, view: 895, Q&A: 0
    The striatum is located in the subcortical region of the forebrain, it contains medium spiny neurons, cholinergic interneurons and GABAergic interneurons, and receives dopaminergic projection in the nigrostriatal pathway. This protocol provides a ...
    Isolation of Murine Brain and Lung Microvascular Endothelial Cells
    Authors:  Ailing Zhang, Shogo Sawaguchi, Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 08/20/2018, view: 1397, Q&A: 0
    This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak et al., 2010; Ruck et al., 2014). We have used the protocol to analyze ...
    Combinations of Patch-Clamp and Confocal Calcium Imaging in Acutely Isolated Adult Mouse Amygdala Brain Slices
    Authors:  Erin E. Koffman and Jianyang Du, date: 08/05/2018, view: 1897, Q&A: 0
    Calcium imaging is a powerful technique in the study of neuronal physiology, as it avoids the enzyme treatment on neurons and is able to study the neuronal activities in vivo. Using calcium-imaging techniques, we are able to monitor the ...
    Protocol for the Isolation and Super-resolution dSTORM Imaging of RyR2 in Cardiac Myocytes
    Authors:  Yufeng Hou, Christopher Le, Christian Soeller and William E. Louch, date: 08/05/2018, view: 1693, Q&A: 0
    Since its inception, super-resolution microscopy has played an increasingly important role in the discovery and characterization of nanoscale biological structure. dSTORM, which is one of the most commonly applied methods, relies on stochastic ...