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    A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
    Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 791, Q&A: 0
    This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
    Phenotypic Profiling of Candida glabrata in Liquid Media
    Authors:  Fabian Istel, Miha Tome, Sabrina Jenull and Karl Kuchler, date: 04/05/2017, view: 1881, Q&A: 0
    Here, we describe a method for a large-scale liquid screening approach in C. glabrata. This liquid media method offers several distinct advantages over solid media approaches. This includes growth measurement on a plate reader instead of ...
    Purification of Adenovirus by Cesium Chloride Density Gradients
    Author:  Huan Pang, date: 04/05/2012, view: 21177, Q&A: 1
    Adenovirus are efficient gene delivery systems. The standard method for purification of adenoviral vectors is based on using a cesium chloride (CsCl) density gradient combined with ultracentrifugation. This method is suitable for ...
    The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells
    Author:  Hogune Im, date: 10/20/2011, view: 13323, Q&A: 1
    This protocol differs from other procedures in that the bacterial culture is grown at 18 °C rather than the conventional 37 °C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Why growing the cells at low temperature ...
    Expression and Purification of GST-tagged Proteins from E. coli
    Author:  Lin Fang, date: 09/20/2011, view: 12888, Q&A: 1
    This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
    qPCR of Yeast ChIP DNA
    Author:  Wei Zheng, date: 09/20/2011, view: 10407, Q&A: 1
    This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample ...
    Yeast in situ Hybridization
    Author:  Wei Zheng, date: 09/20/2011, view: 6579, Q&A: 0
    This protocol describes the procedure to label a certain mRNA species in yeast cells with fluorescence probes and prepare slides for visualization under microscope. Probe design/synthesis and microscopy operation are not covered.
    E. coli Genomic DNA Extraction
    Author:  Fanglian He, date: 07/20/2011, view: 106787, Q&A: 48
    This protocol uses phenol/chloroform method to purify genomic DNA without using commercial kits.
    Making Yeast Competent Cells and Yeast Cell Transformation
    Author:  Yongxian Lu, date: 07/20/2011, view: 25941, Q&A: 2
    This is a quite simple but reliable protocol to make very high transformation efficiency yeast competent cells. By express your gene of interest, protein function can be studied in yeast cells.