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    Cryopreservation of Human Serum
    Authors:  Yael Rosenberg-Hasson and Holden Maecker, date: 11/05/2015, view: 2769, Q&A: 0
    This protocol describes how to collect and aliquot human serum and store at -80 °C for future cytokine/chemokine/protein profile analysis.
    Please use personal protective equipment (PPE) as required when handling samples with potential ...
    Site Density Protocol
    Author:  Jun Huang, date: 07/05/2012, view: 5008, Q&A: 0
    The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T ...
    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
    Author:  Zheng Liu, date: 06/20/2012, view: 7076, Q&A: 0
    Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
    ELISA Measurement of Mouse IL-2
    Author:  Guo N. Huang, date: 01/05/2012, view: 6780, Q&A: 0
    Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...
    Immunofluorescence on Frozen Tissue Sections
    Author:  Jason Reuter, date: 12/20/2011, view: 15322, Q&A: 0
    Immunofluorescence is commonly used to determine the cellular or tissue localization of a protein of interest. Immunofluorescence can also be used as a qualitative measure of protein expression.
    In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
    Author:  Jia Li, date: 11/20/2011, view: 36745, Q&A: 17
    In vitro differentiation of helper T cells of various lineages is frequently used in T helper cell study. Naïve CD4 T cells can differentiate into certain lineage of T help cells in vitro in the presence of specific stimulatory ...
    Naïve T Lymphocyte Infection by Murine Stem Cell Virus (MSCV) Protocol
    Author:  Jia Li, date: 11/20/2011, view: 8317, Q&A: 0
    This assay can be used for studying genes related to lymphocyte proliferation and differentiation in vitro. Stimulating lymphocytes to proliferate is important for the infection efficiency of MSCV. After overnight culture, if naïve cells ...
    Thioglycollate Induced Peritonitis
    Author:  Zheng Liu, date: 06/20/2011, view: 22299, Q&A: 3
    Intraperitoneal (i.p.) injection of thioglycollate elicits a robust influx of neutrophils into peritoneal cavity. The trafficking of the cells is believed to be mediated by chemokines CXCL1, CXCL2, and CXCL8 (Call et al., 2001; Cacalano et ...
    In vivo BrdU Incorporation and Detection in Mouse
    Author:  Zheng Liu, date: 06/05/2011, view: 24728, Q&A: 0
    BrdU (Bromodeoxyuridine or 5-bromo-2’-deoxyuridine) is a synthetic nucleoside that is incorporated into DNA by proliferating cells. This protocol is to be used to incorporate and detect BrdU in murine plasma cells. The plasma cells described in this ...
    Whole Blood Staining of Human Monocyte Subsets for Flow Cytometry
    Author:  Zheng Liu, date: 05/20/2011, view: 27390, Q&A: 10
    This is a general protocol to stain whole human blood for flow analysis with minimal spontaneous activation of monocytes. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.