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Molecular Biology
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Isolation and Maintenance of Striatal Neurons
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Growing and Pollinating Maize
Authors:
Sarah Hake
and
China Lunde
,
date:
05/05/2018,
view:
99,
Q&A:
0
[Abstract]
Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...
Nuclear Transformation of
Chlamydomonas reinhardtii
by Electroporation
Author:
Tyler M Wittkopp
,
date:
05/05/2018,
view:
171,
Q&A:
0
The unicellular green alga
Chlamydomonas reinhardtii
is an important model organism for studying photosynthesis, acclimation to abiotic stress, cilia biology, and many other biological processes. Many molecular biology tools exist for ...
Isolation of Genomic DNA from
Chlamydomonas reinhardtii
Author:
Tyler M Wittkopp
,
date:
05/05/2018,
view:
97,
Q&A:
0
Chlamydomonas reinhardtii
is a soil-dwelling eukaryotic green alga that is widely used as a laboratory model organism for research on photosynthesis, ciliary biology, lipid metabolism and many other aspects of cell biology and physiology. ...
Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
Authors:
Kirsten A. Copren
,
Oyang Teng
,
Cassandra J. Adams
,
Cláudia Bispo
and
Michael Lee
,
date:
04/20/2018,
view:
208,
Q&A:
0
The TaqMan Gene Expression Cells-to-Ct
TM
Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-10
5
cells, though the ...
Small-scale DNA Extraction Method for Maize and Other Plants
Author:
China Lunde
,
date:
03/20/2018,
view:
246,
Q&A:
0
Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model,
Arabidopsis
, see Lunde and Hake, 2005. For an ...
RNA Extraction from Wistar Rat Cochlea for qRT-PCR
Authors:
Janaína Cândida Rodrigues
and
Rubens Vuono de Brito Neto
,
date:
12/05/2017,
view:
780,
Q&A:
0
Otology research has developed considerably in recent years and molecular analysis is crucial to identify metabolic pathways and therapeutic targets. However, the structure of the cochlea limits the amount of cell mass, and special care is required ...
Alkaline Phosphatase
Author:
Fanglian He
,
date:
07/20/2012,
view:
15487,
Q&A:
2
Alkaline phosphatase removes 5' phosphate groups from vector so that prevents self-ligation of the vector and facilitates the ligation of other DNA fragments into the vector.
Protocol for knockdown of HuR with siRNA
Author:
FengZhi Liu
,
date:
05/20/2012,
view:
7883,
Q&A:
1
Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
Cell Culture Mycoplasma Detection by PCR
Author:
Huan Pang
,
date:
04/20/2012,
view:
9939,
Q&A:
0
DNA was extracted from the supernatant of each sample. After PCR-amplification of mycoplasma DNA, detection was performed by gel electrophresis. The PCR primers were designed to cover the consensus sequences that can detect all types of mycoplasma ...
Transfection of S2 Cell with DNA Using CellFectin Reagent
Author:
Darran G. Cronshaw
,
date:
03/05/2012,
view:
13728,
Q&A:
0
This method provides a step-by-step guide to transfecting Drosophila S2 cells with the pRmHA-3 (or similar) vector with insert of choice (in this case SDF-1β-FLAG) and generating a stable cell line. This cell line is then capable of producing the ...