Molecular Biology


    RNA Extraction from Wistar Rat Cochlea for qRT-PCR
    Authors:  Janaína Cândida Rodrigues and Rubens Vuono de Brito Neto, date: 12/05/2017, view: 406, Q&A: 0
    Otology research has developed considerably in recent years and molecular analysis is crucial to identify metabolic pathways and therapeutic targets. However, the structure of the cochlea limits the amount of cell mass, and special care is required ...
    Alkaline Phosphatase
    Author:  Fanglian He, date: 07/20/2012, view: 14249, Q&A: 2
    Alkaline phosphatase removes 5' phosphate groups from vector so that prevents self-ligation of the vector and facilitates the ligation of other DNA fragments into the vector.
    Protocol for knockdown of HuR with siRNA
    Author:  FengZhi Liu, date: 05/20/2012, view: 7659, Q&A: 1
    Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
    Cell Culture Mycoplasma Detection by PCR
    Author:  Huan Pang, date: 04/20/2012, view: 9445, Q&A: 0
    DNA was extracted from the supernatant of each sample. After PCR-amplification of mycoplasma DNA, detection was performed by gel electrophresis. The PCR primers were designed to cover the consensus sequences that can detect all types of mycoplasma ...
    Transfection of S2 Cell with DNA Using CellFectin Reagent
    Author:  Darran G. Cronshaw, date: 03/05/2012, view: 13289, Q&A: 0
    This method provides a step-by-step guide to transfecting Drosophila S2 cells with the pRmHA-3 (or similar) vector with insert of choice (in this case SDF-1β-FLAG) and generating a stable cell line. This cell line is then capable of producing the ...
    Zebrafish Embryo DNA Preparation
    Author:  Lili Jing, date: 02/20/2012, view: 12655, Q&A: 0
    This protocol explains how to extract DNA from a single zebrafish embryo. It does not require the use of expensive kits.
    Genotyping for Single Zebrafish (Fin Clip) or Zebrafish Embryo
    Author:  Lili Jing, date: 02/05/2012, view: 16277, Q&A: 0
    Zebrafish is increasingly used a genetic model organism in biomedical studies. This protocol provides a detailed procedure about the identification of the genotype of an adult zebrafish or a zebrafish embryo.
    Genotyping Transgenic Zebrafish Using Genomic DNA Extracted from Clutch of Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 9505, Q&A: 0
    Transgenic zebrafish are very useful genetic tools to study various biological processes. Identification the right transgene founder and the subsequent transgenic animals are always tedious and time consuming. This protocol provides a relatively ...
    Preparation of Torula Yeast RNA for Hybe Solutions
    Author:  Lili Jing, date: 02/05/2012, view: 13051, Q&A: 5
    In situ hybridization in zebrafish embryos frequently suffers from high background signal. Torular yeast RNA is often added to reduce the non-specific binding that leads to the high background signal. Without appropriate preparation, ...
    In situ Hybridization in Zebrafish Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 8412, Q&A: 0
    In situ hybridization is routinely used to examine the gene expression level and location of embryos. This protocol is modified from the Thisse protocol and is a detailed description of the in situ hybridization procedures in ...