Molecular Biology

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    Unscheduled DNA Synthesis at Sites of Local UV-induced DNA Damage to Quantify Global Genome Nucleotide Excision Repair Activity in Human Cells
    Authors:  Paula J. van der Meer, Diana van den Heuvel and Martijn S. Luijsterburg, date: 02/05/2023, view: 148, Q&A: 0

    Nucleotide excision repair (NER) removes a wide variety of structurally unrelated lesions from the genome, including UV-induced photolesions such as 6–4 pyrimidine–pyrimidone photoproducts (6-4PPs) and cyclobutane pyrimidine dimers

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    A CRISPR-based Strategy for Temporally Controlled Site-Specific Editing of RNA Modifications
    Authors:  Ying Xu, Yufan Wang and Fu-Sen Liang, date: 02/05/2023, view: 33, Q&A: 0

    Chemical modifications on RNA play important roles in regulating its fate and various biological activities. However, the impact of RNA modifications varies depending on their locations on different transcripts and cells/tissues contexts; available

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    Isolation of Nuclei from Human Snap-frozen Liver Tissue for Single-nucleus RNA Sequencing

    Single-nucleus RNA sequencing (snRNA-seq) provides a powerful tool for studying cell type composition in heterogenous tissues. The liver is a vital organ composed of a diverse set of cell types; thus, single-cell technologies could greatly

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    High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS
    Authors:  Hironori Saito, Tatsuya Osaki, Yoshiho Ikeuchi and Shintaro Iwasaki, date: 02/05/2023, view: 79, Q&A: 0

    In addition to cytosolic protein synthesis, mitochondria also utilize another translation system that is tailored for mRNAs encoded in the mitochondrial genome. The importance of mitochondrial protein synthesis has been exemplified by the diverse

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    Preparation and Characterization of DNA-assembled GRS-DNA-CuS Nanodandelions
    Authors:  Haoze Wang, Yanna Cui, Yongming Zhang and Zeyu Xiao, date: 01/20/2023, view: 205, Q&A: 0

    In this study, we introduce a detailed protocol for the preparation of DNA-assembled GRS-DNA-copper sulfide (CuS) nanodandelion, a multifunctional theranostics nanoparticle. Using transmission electron microscope (TEM) and dynamic light

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    Preparation of Caenorhabditis elegans for Scoring of Muscle-derived Exophers
    Authors:  Katarzyna Banasiak, Michał Turek and Wojciech Pokrzywa, date: 01/05/2023, view: 290, Q&A: 0

    Utilizingresources available from the mother's body to guarantee healthy offspring growth is the fundamental reproductive strategy. Recently, we showed that a class of the largest extracellular vesicles known as exophers, which are responsible

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    Staining and Scanning Protocol for Micro-Computed Tomography to Observe the Morphology of Soft Tissues in Ambrosia Beetles

    Advances in imaging technology offer new opportunities in developmental biology. To observe the development of internal structures, microtome cross-sectioning followed by H&E staining on glass slides is a common procedure; however, this

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    BONCAT-based Profiling of Nascent Small and Alternative Open Reading Frame-encoded Proteins
    Authors:  Xiongwen Cao, Yanran Chen, Alexandra Khitun and Sarah A. Slavoff, date: 01/05/2023, view: 255, Q&A: 0

    RIBO-seq and proteogenomics have revealed that mammalian genomes harbor thousands of unannotated small and alternative open reading frames (smORFs, <100 amino acids, and alt-ORFs, >100 amino acids, respectively). Several dozen mammalian

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    Sclerotinia sclerotiorum Protoplast Preparation and Transformation
    Authors:  Chi Lan, Lulu Qiao and Dongdong Niu, date: 01/05/2023, view: 246, Q&A: 1

    Sclerotinia sclerotiorum causes white mold, leading to substantial losses on a wide variety of hosts around the world. Many genes encoding effector proteins play important roles in the pathogenesis of S. sclerotiorum. Therefore, establishment of a

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