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Molecular Biology
Latest Protocol Video
Mating and Tetrad Dissection in
Chlamydomonas
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Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
Authors:
Xuhuai Ji
,
Igor Goncharov
,
Miao Zhao
,
Molly Miranda
and
Holden T. Maecker
,
date:
02/05/2019,
view:
641,
Q&A:
0
Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
Agrobacterium
-mediated Transformation of
Nicotiana tabacum
cv. Xanthi Leaf Explants
Authors:
Ana Lilia Rosales-Campos
and
Abel Gutiérrez-Ortega
,
date:
01/20/2019,
view:
763,
Q&A:
0
Plant transformation has become an important technology for the large-scale production of a number of goods, ranging from vaccines and pharmaceutical products to biofuel. A plant that is frequently used for this purpose is
Nicotiana tabacum
...
Generation of
Fusarium graminearum
Knockout Mutants by the Split-marker Recombination Approach
Authors:
Wan-Qiu Wang
and
Wei-Hua Tang
,
date:
08/20/2018,
view:
1198,
Q&A:
0
Fusarium graminearum
is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by ...
High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
Authors:
Akira Karasawa
and
Toshimitsu Kawate
,
date:
07/20/2018,
view:
1629,
Q&A:
0
P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na
+
...
Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in
Caenorhabditis elegans
Authors:
Alina C. Teuscher
and
Collin Y. Ewald
,
date:
07/20/2018,
view:
3006,
Q&A:
0
Green fluorescent protein (GFP) is widely used as a molecular tool to assess protein expression and localization. In
C. elegans
, the signal from weakly expressed GFP fusion proteins is masked by autofluorescence emitted from the intestinal ...
Plasmid Extract from Budding Yeast (
Saccharomyces cerevisiae
)
Author:
Gal Haimovich
,
date:
07/20/2018,
view:
1774,
Q&A:
0
Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in
Arabidopsis
Authors:
Ziwei Li
,
Songxiao Zhong
,
Wenye Wu
and
Binglian Zheng
,
date:
07/05/2018,
view:
1678,
Q&A:
0
RNA immunoprecipitation (RIP) is an antibody-based technique used to map
in vivo
RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
Phenol-based Extraction of RNA from
Chlamydomonas reinhardtii
Authors:
Emanuel Sanz-Luque
and
Amaury de Montaigu
,
date:
07/05/2018,
view:
1487,
Q&A:
0
RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in
Chlamydomonas
. However, in some cases RNA quality and quantity may be ...
In vitro
Chaperone Activity Assay Using α-Amylase as Target Protein
Authors:
Jeeshma Nambidi Parambath
,
Gayathri Valsala
,
Karthik Menon
and
Shiburaj Sugathan
,
date:
06/20/2018,
view:
1928,
Q&A:
0
Small heat shock proteins (sHSP) are stress proteins which are ubiquitously found in almost all living organisms. They function as molecular chaperones, which assist in protein folding during translation and in the prevention of irreversible protein ...
Total RNA Extraction from Dinoflagellate
Symbiodinium
Cells
Author:
Tingting Xiang
,
date:
06/05/2018,
view:
1202,
Q&A:
1
Dinoflagellates are unicellular algae that can have photosynthetic or nonphotosynthetic lifestyles. Dinoflagellates in the genus
Symbiodinium
can enter endosymbiotic associations with corals, providing the metabolic basis for the highly ...