Worms

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    Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans
    Authors:  Alina C. Teuscher and Collin Y. Ewald, date: 07/20/2018, view: 3283, Q&A: 0
    Green fluorescent protein (GFP) is widely used as a molecular tool to assess protein expression and localization. In C. elegans, the signal from weakly expressed GFP fusion proteins is masked by autofluorescence emitted from the intestinal ...
    Oil Red O Staining of Fixed Worm
    Author:  Fanglian He, date: 07/05/2012, view: 19234, Q&A: 1
    Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco.
    DAPI Nuclear Staining of Live Worm
    Author:  Fanglian He, date: 06/05/2011, view: 14810, Q&A: 0
    Adapted from the Villenueve Lab at Stanford University. This is a very simple method using ethanol fixation, but works very well.
    Making Males of C. elegans
    Author:  Fanglian He, date: 04/20/2011, view: 21487, Q&A: 3
    Getting males from a hermaphrodite population. This is a modified version of protocol originally written by Michael Koelle at Yale University.
    Common Worm Media and Buffers
    Author:  Fanglian He, date: 04/05/2011, view: 38080, Q&A: 14
    Here are recipes of some media and solutions often used in C. elegans research.
    Lifespan Assay
    Author:  Fanglian He, date: 04/05/2011, view: 21460, Q&A: 3
    This assay is used to address aging-related questions in worms.
    Synchronization of Worm
    Author:  Fanglian He, date: 04/05/2011, view: 19055, Q&A: 1
    Attaining a large synchronized population of worms is desirable for use in some assays in order to eliminate variation in results due to age differences. Two ways to get synchronized worms include egg preparation via bleaching and egg lay. The ...