Mammalia

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Western Blot in Maize

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    Cellular Retention Assay to Determine the Interaction Frequency of CD19-directed Chimeric Antigen Receptor (CAR) Engineered Cells against CD19+ Leukemic Cells
    Authors:  Marvin I. De los Santos and Samuel D. Bernal, date: 09/05/2019, view: 74, Q&A: 0
    Cancer recognition by chimeric antigen receptor T (CAR-T) cell is prerequisite for cancer killing to occur. Anomalies in the binding specificity of scFv in the CAR construct may prevent successful eradication of these cancer cells. In some cases, ...
    Dual Fluorescence Reporter Based Analytical Flow Cytometry for miRNA Induced Regulation in Mammalian Cells
    Authors:  Nicolas Lemus-Diaz, Liezel Tamon and Jens Gruber, date: 09/05/2018, view: 3815, Q&A: 0
    MicroRNA-induced gene regulation is a growing field in basic and translational research. Examining this regulation directly in cells is necessary to validate high-throughput data originated from RNA sequencing technologies. For this several studies ...
    High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
    Authors:  Akira Karasawa and Toshimitsu Kawate, date: 07/20/2018, view: 2293, Q&A: 0
    P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na+ ...
    Immunofluorescence Detection and F-actin Staining of MTLn3 Cells
    Author:  Huan Pang, date: 04/20/2012, view: 12570, Q&A: 0
    Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...
    PI (phosphoinositide)-3 Kinase Assay
    Author:  Huan Pang, date: 04/20/2012, view: 8683, Q&A: 1
    PI3-kinases regulate a wide range of cellular responses through the production of phosphatidylinositol 3, 4, 5-trisphosphate [PI (3, 4, 5) P (3)] in cellular membranes. This protocol provides a highly efficient and easy-to-handle method for ...
    Calcium Imaging
    Author:  Mingye Feng, date: 04/05/2012, view: 29700, Q&A: 5
    This is a protocol of Ca2+ imaging experiment using Ca2+ indicator Fura-2. Ca2+ imaging is an efficient and quantitative method for measuring cytosolic and internal store Ca2+ levels, as well as their ...
    Cell Adhesion Assay
    Author:  Yanling Chen, date: 03/05/2012, view: 51559, Q&A: 1
    Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a ...
    Tumor Cell Invasion Assay
    Author:  Yanling Chen, date: 02/05/2012, view: 29975, Q&A: 0
    Cell invasion assays have been used to study the interactions between tumor cells and the extracellular matrix (ECM), which not only provides a structural scaffold for the cell, but also contains various biological factors for the survival and ...
    Calcium Phosphate Transfection of Eukaryotic Cells
    Author:  Yanling Chen, date: 02/05/2012, view: 48357, Q&A: 3
    Transfection of DNA into cells is an indispensible protocol in molecular biology. While plenty of lipid-based transfection reagents are commercially available nowadays, a quick, simple, efficient and inexpensive method is to transfect eukaryotic ...
    Cell Culture Transfection for Production and Purification of Wnt Ligands
    Author:  Lili Jing, date: 01/20/2012, view: 15727, Q&A: 0
    Wnt ligand proteins are extremely difficult to purify and enrich in vitro. This protocol uses Wnt11r protein as an example to illustrate how to use 293T cells to produce secreted Wnt11r and collect it in vitro for further ...