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Western Blot in Maize

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    Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
    Authors:  Jing-Han Liu, Ya-zhong Wang, Lin Chen and Yan He, date: 06/20/2019, view: 264, Q&A: 0
    Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
    Western Blot in Maize
    Author:  María Jazmín Abraham-Juárez, date: 06/05/2019, view: 466, Q&A: 0
    Since its introduction in 1979 (Towbin et al., 1979), protein blotting (Western blot) has become a routine tool in molecular biology research. It is used to detect low amounts of proteins in complex samples or to monitor protein expression ...
    Protein Immunoprecipitation in Maize
    Author:  María Jazmín Abraham-Juárez, date: 06/05/2019, view: 235, Q&A: 0
    Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...
    Hormone Treatments during Maize Vegetative and Reproductive Development
    Authors:  María Jazmín Abraham-Juárez and Harry Klein, date: 05/20/2019, view: 295, Q&A: 0
    Plant hormones are a group of organic natural compounds that can influence physiological processes when present at low concentrations. Plant biologists frequently use exogenous hormone application to modify plant physiology or to rescue mutants with ...
    Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
    Authors:  Jarrett Man and Madelaine E. Bartlett, date: 05/05/2019, view: 667, Q&A: 0
    CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...
    Growing and Pollinating Maize
    Authors:  Sarah Hake and China Lunde, date: 05/05/2018, view: 1994, Q&A: 0
    [Abstract] Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...