Mammalia

Latest Protocol Video

Western Blot in Maize

Categories

    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 1642, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    RNP-IP (Original Method): Obtaining Majority RNA from RNA Binding Protein in the Nucleus
    Author:  FengZhi Liu, date: 05/20/2012, view: 16250, Q&A: 1
    Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and ...
    Protocol for knockdown of HuR with siRNA
    Author:  FengZhi Liu, date: 05/20/2012, view: 9392, Q&A: 1
    Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
    Cell Culture Mycoplasma Detection by PCR
    Author:  Huan Pang, date: 04/20/2012, view: 14131, Q&A: 0
    DNA was extracted from the supernatant of each sample. After PCR-amplification of mycoplasma DNA, detection was performed by gel electrophresis. The PCR primers were designed to cover the consensus sequences that can detect all types of mycoplasma ...
    Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
    Author:  Hui Zhu, date: 04/05/2012, view: 19423, Q&A: 3
    Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell ...
    Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
    Author:  Hui Zhu, date: 04/05/2012, view: 54486, Q&A: 2
    Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
    Protocol to Determine Mitotic Index by FACS
    Author:  Hui Zhu, date: 03/20/2012, view: 14872, Q&A: 0
    Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...
    Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation
    Author:  Hui Zhu, date: 02/05/2012, view: 15888, Q&A: 0
    Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively ...
    Sucrose Gradient Analysis of Proteins
    Author:  Hui Zhu, date: 02/05/2012, view: 23371, Q&A: 1
    The sedimentation rate of a protein in a linear sucrose gradient can be used to determine its S value when compared to proteins of known S values which are run in a parallel gradient. This can be used to estimate an approximate molecular weight of ...
    Restriction Enzyme Accessibility Protocol in Mammalian Cells
    Author:  Hogune Im, date: 10/20/2011, view: 9073, Q&A: 0
    This protocol describes a method to indirectly assess chromatin structure by using restriction enzyme in mammalian cells, modified from Current Protocols.