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Western Blot in Maize

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    A Simplified Hydroponic Culture of Arabidopsis
    Authors:  Houqing Zeng, Chao Xia, Cankui Zhang and Li-Qing Chen, date: 12/20/2018, view: 1432, Q&A: 0
    Hydroponic culture systems are widely used in research due to their intrinsic properties, such as easily altering nutrient composition, applying chemical treatments like metal, salt and hormones in the growth media, and collecting the root sample. ...
    Quantification of Root Colonizing Bacteria
    Authors:  Maged M. Saad, Axel de Zélicourt, Eleonora Rolli , Lukas Synek and Heribert Hirt , date: 07/20/2018, view: 1167, Q&A: 0
    Here we describe a simple method to quantify the number of viable bacteria (e.g., Enterobacter sp. SA187) that colonize the root system of Arabidopsis thaliana.
    Arabidopsis Growing Protocol – A General Guide
    Author:  Xiyan Li, date: 09/05/2011, view: 23819, Q&A: 1
    Arabidopsis as the model organism for higher plants is widely studied among plant biology labs around the world. However, taking care of this tiny plant may not be trivial. Here is a general guide used for the Heven Sze lab at the University of ...
    Extract Genomic DNA from Arabidopsis Leaves (Can be Used for Other Tissues as Well)
    Author:  Yongxian Lu, date: 07/05/2011, view: 23473, Q&A: 1
    This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.
    Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis
    Author:  Xiyan Li, date: 07/05/2011, view: 22893, Q&A: 3
    Promoter-driven GUS (beta-glucuronidase) activity is the most commonly used technique for tissue-specific expression patterns in Arabidopsis. In this procedure, GUS enzyme converts 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc) to a blue product. ...
    DNA Extraction from Dried Plant Tissues Using 96-well Format (cTab Method)
    Author:  Yongxian Lu, date: 07/05/2011, view: 14341, Q&A: 5
    This high throughput DNA isolation protocol is used to extract DNA of high quality from plant tissues for various genetics studies, like genotyping, and mapping etc. This protocol uses the well-established CTAB extraction procedure, and has been ...
    Simplified Agrobacterium-mediated Transformation of Arabidopsis thaliana
    Author:  Li-Qing Chen, date: 03/05/2011, view: 16356, Q&A: 0
    Acquiring transgenic lines is fundamental strategy for plant gene/protein function study. How to successfully obtain the transgenic lines is a big matter with transformation methods. Now Arabidopsis floral dip transformation method is more ...