A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays
    Authors:  Michael D. Caponegro, Kaitlyn Koenig Thompson, Maryam Tayyab and Stella E. Tsirka, date: 08/05/2020, view: 400, Q&A: 0
    Studying monocytic cells in isolated systems in vitro contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as ...
    Striatal Synaptosomes Preparation from Mouse Brain
    Authors:  Shiqi Ma and Alexander Sorkin, date: 09/05/2018, view: 2319, Q&A: 0
    The striatum is located in the subcortical region of the forebrain, it contains medium spiny neurons, cholinergic interneurons and GABAergic interneurons, and receives dopaminergic projection in the nigrostriatal pathway. This protocol provides a ...
    Isolation of Murine Brain and Lung Microvascular Endothelial Cells
    Authors:  Ailing Zhang, Shogo Sawaguchi, Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 08/20/2018, view: 2884, Q&A: 0
    This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak et al., 2010; Ruck et al., 2014). We have used the protocol to analyze ...
    Combinations of Patch-Clamp and Confocal Calcium Imaging in Acutely Isolated Adult Mouse Amygdala Brain Slices
    Authors:  Erin E. Koffman and Jianyang Du, date: 08/05/2018, view: 4090, Q&A: 0
    Calcium imaging is a powerful technique in the study of neuronal physiology, as it avoids the enzyme treatment on neurons and is able to study the neuronal activities in vivo. Using calcium-imaging techniques, we are able to monitor the ...
    Stereotaxic Injection of LPS or Your Reagents of Choice into Rat Substantia Nigra
    Author:  Hui-Ming Gao, date: 11/20/2011, view: 10237, Q&A: 0
    Stereotaxic injection is an invaluable tool for the creation of site-targeted lesions, injection of anatomical tracers, gene delivery by recombinant adeno-associated viruses and lentiviruses in mice and rats. Stereotaxic injection of LPS or ...
    Generation of Neuron-enriched Cultures (Method 1)
    Author:  Huiming Gao, date: 11/05/2011, view: 9725, Q&A: 0
    This protocol will guide you through the process for generating midbrain neuronal cultures from late embryo mouse brain. These cultures serve as a useful tool to study molecular pathways during neuronal death in various neurological disorders. This ...
    Generation of Neuron-enriched Cultures (Method 2)
    Author:  Huiming Gao, date: 11/05/2011, view: 11601, Q&A: 0
    Neuron-enriched cultures are a useful tool to study neuronal development and the molecular pathways that come in to play during neuronal death in various neurological disorders. This protocol is for generating midbrain neuronal cultures from late ...
    Generation of Microglia Cultures and Mixed Glial Culture
    Author:  Huiming Gao, date: 11/05/2011, view: 17714, Q&A: 3
    Primary rodent microglia-enriched cultures are the most popular model to study microglial biology in vitro and to explore immune signaling pathways. Mixed glial cultures that contain microglia and astroglia are very useful for investigating ...

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