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Western Blot in Maize

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    Ty1 Retrotransposition Frequency Assay Using a Chromosomal Ty1his3AI or Ty1kanMXAI Element
    Author:  M. Joan Curcio, date: 09/05/2018, view: 866, Q&A: 0
    Here I describe a simple genetic assay to determine the frequency of retrotransposition of a single chromosomal Ty1 element that is marked with the retrotransposition indicator gene, his3AI or kanMXAI. The assay is used to ...
    Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
    Author:  Gal Haimovich, date: 07/20/2018, view: 2214, Q&A: 0
    Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
    GST-tagged Yeast Protein Purification
    Author:  Hogune Im, date: 10/05/2011, view: 12824, Q&A: 2
    Glutation S-transferase (GST) tagging is the most commonly used purification strategy for recombinant protein. It was developed with the goal of preserving the enzymatic activity by utilizing gentle elution condition of the target protein from ...
    Polyribosome Profiling
    Author:  Wei Zheng, date: 10/05/2011, view: 9139, Q&A: 0
    qPCR of Yeast ChIP DNA
    Author:  Wei Zheng, date: 09/20/2011, view: 13230, Q&A: 1
    This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample ...
    Yeast in situ Hybridization
    Author:  Wei Zheng, date: 09/20/2011, view: 8858, Q&A: 0
    This protocol describes the procedure to label a certain mRNA species in yeast cells with fluorescence probes and prepare slides for visualization under microscope. Probe design/synthesis and microscopy operation are not covered.
    Making Yeast Competent Cells and Yeast Cell Transformation
    Author:  Yongxian Lu, date: 07/20/2011, view: 33321, Q&A: 3
    This is a quite simple but reliable protocol to make very high transformation efficiency yeast competent cells. By express your gene of interest, protein function can be studied in yeast cells.
    Yeast Transcription Factor Chromatin Immunoprecipitation
    Author:  Wei Zheng, date: 07/05/2011, view: 10505, Q&A: 0
    This ChIP protocol was developed and improved over the years by various researchers in the Snyder lab, Stanford University, especially Anthony Borneman and Christopher Yellman. I have used this method to successfully map the genome-wide binding of ...
    Yeast Genomic DNA Miniprep Using A FastPrep Cell Lyser
    Author:  Xiyan Li, date: 05/05/2011, view: 9438, Q&A: 3
    This method is a convenient way to purify high-quality genomic DNA from yeast cells. It is suitable for PCR and other assays that require genomic DNA of higher quality.