Mammalia

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    Generation of Endothelial Cells from Human Induced Pluripotent Stem Cells
    Authors:  Chun Liu, Linling Cheng, Christopher Li Chen and Nazish Sayed, date: 11/20/2018, view: 1079, Q&A: 4
    Endothelial cells (ECs), as important constituent of blood vessel are crucial to maintaining vascular tone, and any dysfunction in ECs could lead to many diseases of the vascular system. Due to limited availability of human samples, most of our ...
    High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
    Authors:  Akira Karasawa and Toshimitsu Kawate, date: 07/20/2018, view: 1778, Q&A: 0
    P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na+ ...
    Cryopreservation of Human Serum
    Authors:  Yael Rosenberg-Hasson and Holden Maecker, date: 11/05/2015, view: 4761, Q&A: 0
    This protocol describes how to collect and aliquot human serum and store at -80 °C for future cytokine/chemokine/protein profile analysis.
    Please use personal protective equipment (PPE) as required when handling samples with potential ...
    Immunofluorescence Detection and F-actin Staining of MTLn3 Cells
    Author:  Huan Pang, date: 04/20/2012, view: 12042, Q&A: 0
    Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...
    Make Human Umbilical Vein Endothelial Cells from Cords
    Author:  Huan Pang, date: 04/20/2012, view: 9956, Q&A: 0
    Human umbilical vein endothelial cells (HUVEC) can be isolated from normal human umbilical veins, and are responsive to cytokine stimulation by expressing cell adhesion molecules. These cell systems are commonly used for physiological and ...
    PI (phosphoinositide)-3 Kinase Assay
    Author:  Huan Pang, date: 04/20/2012, view: 8295, Q&A: 1
    PI3-kinases regulate a wide range of cellular responses through the production of phosphatidylinositol 3, 4, 5-trisphosphate [PI (3, 4, 5) P (3)] in cellular membranes. This protocol provides a highly efficient and easy-to-handle method for ...
    Calcium Imaging
    Author:  Mingye Feng, date: 04/05/2012, view: 28377, Q&A: 5
    This is a protocol of Ca2+ imaging experiment using Ca2+ indicator Fura-2. Ca2+ imaging is an efficient and quantitative method for measuring cytosolic and internal store Ca2+ levels, as well as their ...
    Cell Adhesion Assay
    Author:  Yanling Chen, date: 03/05/2012, view: 48663, Q&A: 1
    Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a ...
    Tumor Cell Invasion Assay
    Author:  Yanling Chen, date: 02/05/2012, view: 28149, Q&A: 0
    Cell invasion assays have been used to study the interactions between tumor cells and the extracellular matrix (ECM), which not only provides a structural scaffold for the cell, but also contains various biological factors for the survival and ...
    Calcium Phosphate Transfection of Eukaryotic Cells
    Author:  Yanling Chen, date: 02/05/2012, view: 44517, Q&A: 3
    Transfection of DNA into cells is an indispensible protocol in molecular biology. While plenty of lipid-based transfection reagents are commercially available nowadays, a quick, simple, efficient and inexpensive method is to transfect eukaryotic ...