Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans
    Authors:  Alina C. Teuscher and Collin Y. Ewald, date: 07/20/2018, view: 6579, Q&A: 0
    Green fluorescent protein (GFP) is widely used as a molecular tool to assess protein expression and localization. In C. elegans, the signal from weakly expressed GFP fusion proteins is masked by autofluorescence emitted from the intestinal ...
    Morphological Analysis of Dopaminergic Neurons with Age Using Caenorhabditis elegans GFP Reporter Strains
    Authors:  Sanjib Guha, Guy Caldwell and Pankaj Kapahi, date: 05/05/2018, view: 4025, Q&A: 0
    This protocol describes how to quantify different morphological defects as observed in the dopaminergic neurons using C. elegans GFP reporter strains with age.
    Oil Red O Staining of Fixed Worm
    Author:  Fanglian He, date: 07/05/2012, view: 22947, Q&A: 1
    Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco.
    Expansion of Worms for Microarray, IP, ChIP and Similar Experiments
    Author:  Peichuan Zhang, date: 09/05/2011, view: 10657, Q&A: 1
    This protocol describes the basic work-flow of expanding nematode culture under lab conditions, which serves for the subsequent preparation of RNA (microarray), protein (IP), and DNA/protein (ChIP).
    DAPI Nuclear Staining of Live Worm
    Author:  Fanglian He, date: 06/05/2011, view: 17057, Q&A: 0
    Adapted from the Villenueve Lab at Stanford University. This is a very simple method using ethanol fixation, but works very well.
    Single Worm PCR
    Author:  Fanglian He, date: 04/20/2011, view: 18795, Q&A: 1
    This protocol is used for genotyping single worms.
    RNA Interference (RNAi) by Bacterial Feeding
    Author:  Fanglian He, date: 04/20/2011, view: 24837, Q&A: 0
    There are 3 ways to perform RNAi in worms: microinjection, soaking and feeding. In the feeding protocol, RNAi is induced by cultivating worms on bacteria expressing gene-specific dsRNA. dsRNA is expressed in E. coli and ingested by worms. ...
    Making Males of C. elegans
    Author:  Fanglian He, date: 04/20/2011, view: 25894, Q&A: 3
    Getting males from a hermaphrodite population. This is a modified version of protocol originally written by Michael Koelle at Yale University.
    Common Worm Media and Buffers
    Author:  Fanglian He, date: 04/05/2011, view: 46601, Q&A: 15
    Here are recipes of some media and solutions often used in C. elegans research.
    Lifespan Assay
    Author:  Fanglian He, date: 04/05/2011, view: 24867, Q&A: 3
    This assay is used to address aging-related questions in worms.

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