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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Microbiology
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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
Authors:
Ankur Garg
,
Lihong Liu
,
David D. Ho
and
Leemor Joshua-Tor
,
date:
08/05/2020,
view:
287,
Q&A:
0
This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in
Escherichia coli
. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...
Expression and Purification of GST-tagged Proteins from
E. coli
Author:
Lin Fang
,
date:
09/20/2011,
view:
19730,
Q&A:
1
This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
Small Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
Author:
Bio-protocol Editor
,
date:
01/05/2011,
view:
15796,
Q&A:
1
In this protocol, we show how to purify membrane proteins from yeast using affinity purification under native conditions at a small scale.
Large Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
Author:
Bio-protocol Editor
,
date:
01/05/2011,
view:
16972,
Q&A:
0
This protocol can be used to purify membrane proteins from yeast samples under native conditions at a large scale. This protocol has been developed primarily for FLAG-tagged proteins. This protocol can however be slightly modified and applied to ...
Purification of 6x His-tagged Protein (from
E. coli
)
Author:
Bio-protocol Editor
,
date:
01/05/2011,
view:
24900,
Q&A:
1
A polyhistidine-tag is an amino acid motif that contains at least six histidine (His) residues, usually at the N- or C-terminus of the protein. This tag can also be referred to as a hexa histidine-tag or a 6x His-tag. The protocol described here has ...
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