Microbiology

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    Expression and Purification of GST-tagged Proteins from E. coli
    Author:  Lin Fang, date: 09/20/2011, view: 18453, Q&A: 1
    This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
    Small Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 15065, Q&A: 1
    In this protocol, we show how to purify membrane proteins from yeast using affinity purification under native conditions at a small scale.
    Large Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 16069, Q&A: 0
    This protocol can be used to purify membrane proteins from yeast samples under native conditions at a large scale. This protocol has been developed primarily for FLAG-tagged proteins. This protocol can however be slightly modified and applied to ...
    Purification of 6x His-tagged Protein (from E. coli)
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 22590, Q&A: 1
    A polyhistidine-tag is an amino acid motif that contains at least six histidine (His) residues, usually at the N- or C-terminus of the protein. This tag can also be referred to as a hexa histidine-tag or a 6x His-tag. The protocol described here has ...



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