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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice

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    Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
    Authors:  Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor, date: 08/05/2020, view: 287, Q&A: 0
    This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...
    Expression and Purification of GST-tagged Proteins from E. coli
    Author:  Lin Fang, date: 09/20/2011, view: 19730, Q&A: 1
    This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
    Small Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 15796, Q&A: 1
    In this protocol, we show how to purify membrane proteins from yeast using affinity purification under native conditions at a small scale.
    Large Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 16972, Q&A: 0
    This protocol can be used to purify membrane proteins from yeast samples under native conditions at a large scale. This protocol has been developed primarily for FLAG-tagged proteins. This protocol can however be slightly modified and applied to ...
    Purification of 6x His-tagged Protein (from E. coli)
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 24900, Q&A: 1
    A polyhistidine-tag is an amino acid motif that contains at least six histidine (His) residues, usually at the N- or C-terminus of the protein. This tag can also be referred to as a hexa histidine-tag or a 6x His-tag. The protocol described here has ...



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