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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Cancer Biology
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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
Author:
Hui Zhu
,
date:
04/05/2012,
view:
62350,
Q&A:
2
Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
Protocol to Determine Mitotic Index by FACS
Author:
Hui Zhu
,
date:
03/20/2012,
view:
17139,
Q&A:
0
Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...
Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation
Author:
Hui Zhu
,
date:
02/05/2012,
view:
17545,
Q&A:
0
Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively ...
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