Cell Biology



    Preparation of Torula Yeast RNA for Hybe Solutions
    Author:  Lili Jing, date: 02/05/2012, view: 17653, Q&A: 5
    In situ hybridization in zebrafish embryos frequently suffers from high background signal. Torular yeast RNA is often added to reduce the non-specific binding that leads to the high background signal. Without appropriate preparation, ...
    In situ Hybridization in Zebrafish Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 13232, Q&A: 0
    In situ hybridization is routinely used to examine the gene expression level and location of embryos. This protocol is modified from the Thisse protocol and is a detailed description of the in situ hybridization procedures in ...
    Double in situ Hybridization
    Author:  Lili Jing, date: 02/05/2012, view: 14248, Q&A: 0
    Double in situ hybridization I very useful to examine the relationship between the expression of two genes. But it is tricky because of the cross reaction of two different antibodies. This protocol is a valid method to do double color in ...
    DAPI Nuclear Staining of Live Worm
    Author:  Fanglian He, date: 06/05/2011, view: 16851, Q&A: 0
    Adapted from the Villenueve Lab at Stanford University. This is a very simple method using ethanol fixation, but works very well.

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