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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice

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    Protocol for Whole Cell Lysis of Yeast
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 39016, Q&A: 5
    This protocol describes how to perform lysis on whole yeast cell samples using NaOH. The lysed cells can then be used for downstream applications such as the extraction of total proteins.
    Metabolic Labeling of Yeast Proteins
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 12336, Q&A: 0
    Proteins of Saccharomyces cerevisiae can be metabolically labeled with (35) methionine. After labeling, a protocol is described for the mechanical disruption of yeast cells or conversion to spheroplasts, with subsequent lysis before ...
    How to Use an Avestin Emulsiflex C3 Homogenizer to Disrupt Cells
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 20974, Q&A: 1
    The EmulsiFlex-C3 homogenizer is powered by an electric motor. The pump does not require a compressor for it to run. This equipment can be used to disrupt cells at a large scale. The EmulsiFlex-C3 has a fixed flow-through capacity of 3 L/h. It has ...



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