Cell Biology

Latest Protocol Video

Western Blot in Maize

Categories

    A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
    Authors:  Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2018, view: 1075, Q&A: 0
    Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...
    Dissection and Whole Mount Staining of Retina from Neonatal Mice
    Authors:  Mitsutaka Ogawa and Tetsuya Okajima, date: 10/05/2018, view: 1900, Q&A: 0
    Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice (Sawaguchi et al., 2017) by modifying the original protocols (Powner et al., 2012; ...
    Combinations of Patch-Clamp and Confocal Calcium Imaging in Acutely Isolated Adult Mouse Amygdala Brain Slices
    Authors:  Erin E. Koffman and Jianyang Du, date: 08/05/2018, view: 2454, Q&A: 0
    Calcium imaging is a powerful technique in the study of neuronal physiology, as it avoids the enzyme treatment on neurons and is able to study the neuronal activities in vivo. Using calcium-imaging techniques, we are able to monitor the ...
    Protocol for the Isolation and Super-resolution dSTORM Imaging of RyR2 in Cardiac Myocytes
    Authors:  Yufeng Hou, Christopher Le, Christian Soeller and William E. Louch, date: 08/05/2018, view: 2113, Q&A: 0
    Since its inception, super-resolution microscopy has played an increasingly important role in the discovery and characterization of nanoscale biological structure. dSTORM, which is one of the most commonly applied methods, relies on stochastic ...
    Visualization of Growth and Morphology of Fungal Hyphae in planta Using WGA-AF488 and Propidium Iodide Co-staining
    Authors:  Amey Redkar, Elaine Jaeger and Gunther Doehlemann, date: 07/20/2018, view: 2742, Q&A: 0
    Fungal pathogens colonizing plants show a varying degree of symptoms. Microscopy techniques have been used to study the infection and proliferation of fungal hyphae inside the host. One of the best optimized and commonly used method is the ...