Biochemistry

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Western Blot in Maize

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    Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
    Author:  Hui Zhu, date: 04/05/2012, view: 19395, Q&A: 3
    Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell ...
    Cell Culture Transfection for Production and Purification of Wnt Ligands
    Author:  Lili Jing, date: 01/20/2012, view: 15530, Q&A: 0
    Wnt ligand proteins are extremely difficult to purify and enrich in vitro. This protocol uses Wnt11r protein as an example to illustrate how to use 293T cells to produce secreted Wnt11r and collect it in vitro for further ...
    Immunofluorescence on Frozen Tissue Sections
    Author:  Jason Reuter, date: 12/20/2011, view: 22138, Q&A: 0
    Immunofluorescence is commonly used to determine the cellular or tissue localization of a protein of interest. Immunofluorescence can also be used as a qualitative measure of protein expression.