Microbiology

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    A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
    Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 12636, Q&A: 1
    This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
    Purification of Adenovirus by Cesium Chloride Density Gradients
    Author:  Huan Pang, date: 04/05/2012, view: 29527, Q&A: 1
    Adenovirus are efficient gene delivery systems. The standard method for purification of adenoviral vectors is based on using a cesium chloride (CsCl) density gradient combined with ultracentrifugation. This method is suitable for ...
    The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells
    Author:  Hogune Im, date: 10/20/2011, view: 33802, Q&A: 1
    This protocol differs from other procedures in that the bacterial culture is grown at 18 °C rather than the conventional 37 °C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Why growing the cells at low temperature ...
    RbCl Super Competent Cells
    Author:  Xiyan Li, date: 06/05/2011, view: 20245, Q&A: 3
    This method is used to inexpensively prepare home-made competent cells of E. coli. The transformation efficiency is at the high end of chemical-efficient competent cells, and close to library-efficient competent cells.



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