Molecular Biology


    A Detailed and Radioisotope-free Protocol for Electrophoretic Mobility Shift Assay (EMSA)
    Author:  Nguyen Hoai Nguyen, date: 08/20/2020, view: 505, Q&A: 0
    To comprehensively characterize the functions of a transcription factor (TF), it is required to analyze the interaction of this TF with its targeted loci. Several methods such as β-glucuronidase (GUS) or luciferase reporter, yeast one-hybrid (Y1H), ...
    Restriction Enzyme Accessibility Protocol in Mammalian Cells
    Author:  Hogune Im, date: 10/20/2011, view: 10492, Q&A: 0
    This protocol describes a method to indirectly assess chromatin structure by using restriction enzyme in mammalian cells, modified from Current Protocols.
    Yeast Transcription Factor Chromatin Immunoprecipitation
    Author:  Wei Zheng, date: 07/05/2011, view: 12183, Q&A: 0
    This ChIP protocol was developed and improved over the years by various researchers in the Snyder lab, Stanford University, especially Anthony Borneman and Christopher Yellman. I have used this method to successfully map the genome-wide binding of ...
    A General EMSA (Gel-shift) Protocol
    Author:  Ran Chen, date: 02/05/2011, view: 57143, Q&A: 3
    An electrophoretic mobility shift assay (EMSA), also referred to as mobility shift electrophoresis, a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common technique used to study protein-DNA or ...
    ChIP Assay for Cell Culture
    Author:  Ran Chen, date: 01/20/2011, view: 16441, Q&A: 0
    Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. Following crosslinking, cells are lysed and the DNA is broken into pieces 0.2-1.0 kb in length ...

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